Paper Titles in Periodical
International Letters of Chemistry, Physics and Astronomy
ILCPA Volume 60
Subscribe

Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

ILCPA > ILCPA Volume 60 > Validated Spectrophotometric Method to Assay of B6...
Validated Spectrophotometric Method to Assay of B<sub>6</sub> and B<sub>3</sub> Vitamins in Pharmaceutical Forms Using Potassium Iodide and Potassium Iodate
< Back to Volume

Validated Spectrophotometric Method to Assay of B6 and B3 Vitamins in Pharmaceutical Forms Using Potassium Iodide and Potassium Iodate

Full Text PDF

Abstract:

A simple, precise and reliable spectrophotometric method for the determination of pyridoxine hydrochloride (vitB6) and nicotinamide (vitB3) in pure form and pharmaceutical formulations has been described. The method is based on the reaction of studied vitamin with a mixture of potassium iodide and potassium iodate in aqueous medium at (250.5oC) to form yellow colored tri iodide ions (I3-). The reaction is followed spectrophotometrically by measuring the absorbance at 290, 335 and 288, 350 nm for vitB6 and vitB3 respectively. Beer's law was obeyed in the range of 0.5-20.0 μg mL-1 for all procedures. Molar absorptivities were found to be 1.49×104, 1.93×104 and 0.89×104, 1.25×104 L mol-1 cm-1, for determination of vitB6 and vitB3 at 290, 335 and 288, 350 nm respectively. The proposed method has been applied to determine the components in commercial forms with no interference from the excipients. A comparative study between the suggested procedures and the official methods showed no significant difference between the two methods.

Info:

Periodical:
International Letters of Chemistry, Physics and Astronomy (Volume 60)
Pages:
113-119
DOI:
https://doi.org/10.18052/www.scipress.com/ILCPA.60.113
Citation:
M. Khateeb et al., "Validated Spectrophotometric Method to Assay of B6 and B3 Vitamins in Pharmaceutical Forms Using Potassium Iodide and Potassium Iodate", International Letters of Chemistry, Physics and Astronomy, Vol. 60, pp. 113-119, 2015
Online since:
September 2015
Authors:
Mouhammed Khateeb, Basheer Elias, Fatema Al Rahal
Keywords:
Nicotinamide, Pharmaceutical Formulations, Potassium Iodate, Potassium Iodide, Pyridoxine Hydrochloride, Spectrophotometry
Export:
RIS, BibTeX, Text
Distribution:
Open Access

Creative Commons License
This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

British Pharmacopoeia, Her Majesty Stationery Officer, London, UK. (2009).

Muszalska, I Puchalska M; Sobczak A.; Acta Pol. Pharm - Drug Research. 68 (2011) 845.

Abdulkadir A. N.; Rafidain Journal of science. 21 (2010) 49.

Consigliere V. O; Mattavals N.R.; Magalhães, J.F., Anal Lett. 34 (2001) 1875.

Monferrer P. L; Alvarez R. L; Esteve R. J; Garcia A. C M.C.; Anal Lett. 33 (2000) 539.

Portela J. G; Spínola C.A. C; Gomes T.L.S., J Pharmaceut Biomed. 34 (2004) 543.

Ortega A. S; Ramos M. N; Molina D.A.; Spectrosc Lett. 36 (2003) 133.

Qu W; Wu K; Hu S.; J Pharmaceut Biomed. 36 (2004) 631.

Habibi B; Phezhhan H; Pournaghi A M.H., J Iran Chem Soc. 7 (2010) 103.

Teixeiraa M.F. S; Segninia A; Moraesb F. C; Marcolino J. L. H; Fatibello F. O; Cavalheiro É.T.G.; J Brazil Chem Soc. 14 (2003) 316.

Zhang Y; Wang Y.; American Journal of Analytical Chemistry (AJAC). 2 (2011) 194.

Ganjali M.R.; Norouzi P; Shirvani A. S, Mohammadi A., Russian in Elektrokhimiya, 44 (2008) 173.

Vaze V. D; Srivastava A.K.; J Pharmaceut Biomed. 47 (2008) 177.

Mostafa G.A.E.; J Anal Chem. 58 (2003) 1073.

Krasnova A. I; Aguilar C M. P; Gómehens A.; Anal Chim Acta. 441 (2001) 249.

Hassan R. O; Faizullah A.T.; Arabian J Chem. 6 (2013) 393.

Kirchmeierx R. L; Upton R.P.; J Pharm Sci. 67 (1987) (1944).

Li K.; Biomed Chromatogr. 16 (2002) 504.

Amidžtc R; Brboric J; Čudina O; Vladimirovm S.; J Serb Chem Soc 70 (2005) 1229.

Jin P; Xia L; Li Z; Che N; Zou D; Hu X.; J Pharmaceut Biomed. 70 (2012) 151.

Moreno P; Salvadó V.; J Chromatogr A. 870 (2000) 207.

Höller U; Brodhag C, Knöbel A; Hofmann P, Spitzer V.; J Pharmaceut Biomed. 31 (2003) 151.

Chen Z; Chen B; Yao S.; Anal Chim Acta. 569 (2006) 169.

Vazquez R, Le Hoang M. D; Martin J; Ait Yahia Y; Graffard H; Guyon F; Do, B.; European Journal of Hospital Pharmacy Science (EJHP). 15 (2009) 28.

Ringbom A, Zeitschrift für Analytische Chemie. 115 (1939) 332.

Show More Hide
Cited By:

[1] R. Sayed, M. El-Masry, W. Hassan, M. El-Mammli, A. Shalaby, H. Aboul-Enein, "Validated HPLC Method for the simultaneous determination of acyclovir and co-administered vitamin B3 and gabapentin in spiked human plasma", Separation Science Plus, Vol. 1, p. 475, 2018

DOI: https://doi.org/10.1002/sscp.201800040

[2] V. Enemor, C. Oguazu, A. Odiakosa, S. Okafor, " Evaluation of the Medicinal Properties and Possible Nutrient Composition of Citrullus lanatus (Watermelon) Seeds", Research Journal of Medicinal Plants, Vol. 13, p. 129, 2019

DOI: https://doi.org/10.3923/rjmp.2019.129.135

[3] S. Affas, A. Sakur, "Validated green spectrophotometric kinetic method for determination of Clindamycin Hydrochloride in capsules", BMC Chemistry, Vol. 15, 2021

DOI: https://doi.org/10.1186/s13065-021-00755-0

[4] B. Saleem, R. Hamoo, G. Ismail, "Spectrophotometric assay of Nicotinamide in Pharmaceutical Dosages", Journal of Physics: Conference Series, Vol. 2063, p. 012017, 2021

DOI: https://doi.org/10.1088/1742-6596/2063/1/012017

[5] O. Akande, M. Oluwamukomi, O. Osundahunsi, O. Ijarotimi, I. Mukisa, "Evaluating the potential for utilising migratory locust powder (Locusta migratoria) as an alternative protein source in peanut-based ready-to-use therapeutic foods", Food Science and Technology International, p. 108201322110697, 2022

DOI: https://doi.org/10.1177/10820132211069773
Show More Hide