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International Letters of Chemistry, Physics and Astronomy
Volume 50

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New Kinetic Spectrophotometric Method for Determination of Folic Acid in Pharmaceutical Formulations

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Abstract:

A simple and sensitive kinetic spectrophotometric method has been developed for the determination of folic acid (FA) in bulk and pharmaceutical Formulations. The method is based on the oxidation of FA by Fe (III) in sulfuric acid medium. Fe (III) subsequently reduces to Fe (II) which is coupled with potassium ferricyanide to form Prussian blue. The reaction is followed spectrophotometrically by measuring the increase in absorbance at λmax 725 nm. The rate data and fixed time methods were adopted for constructing the calibration curves. The linearity range was found to be 1–20 μg mL-1 for each method. The correlation coefficient was 0.9978 and 0.9993, and LOD was found to be 0.91 and 0.09 μg mL-1 for rate data and fixed time methods, respectively. The proposed method has been successfully applied to the determination of FA in formulations with no interference from the excipients. Statical comparison of the results shows that there is no significant difference between the proposed and pharmacopoeial methods

Info:

Periodical:
International Letters of Chemistry, Physics and Astronomy (Volume 50)
Pages:
169-178
DOI:
https://doi.org/10.18052/www.scipress.com/ILCPA.50.169
Citation:
M. Khateeb et al., "New Kinetic Spectrophotometric Method for Determination of Folic Acid in Pharmaceutical Formulations", International Letters of Chemistry, Physics and Astronomy, Vol. 50, pp. 169-178, 2015
Online since:
May 2015
Authors:
Mouhammed Khateeb, Basheer Elias, Fatema Al Rahal
Keywords:
Folic Acid, Kinetic Spectrophotometry, Pharmaceutical Formulations, Potassium Ferricyanide
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Distribution:
Open Access

Creative Commons License
This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

British Pharmacopoeia, Her Majesty Stationery Officer, London, UK. (2007).

Mangas A, Coveǹas, R Geffard, K Geffard, M Marcos, P Insausti, R Dabadie, M.P., Neurosci Lett. 362 (2004) 258.

Kanjilal G, Mahajan S. N, Ramana R.G., Analyst. 100 (1975) 19.

Padmarajaiah N, Ramanathapura A. V, Hemmige S.Y., Anal Bio chem. 307 (2002) 316.

Zhang B. T, Zhao L, Lin J.M., Talanta. 74 (2008) 1154.

Nie F, He Y, Lu J., Micro chem J. 65 (2000) 319.

Zhang Z. Q, Tang Y., Anal Bio chem. 381(2005) 932.

Al-warthan A.A., Anal Sci. 10 (1994) 919.

Manzoori J. L, Jouyban A, Amjadi M, Soleymani,J., Luminescence. 26 (2011) 106.

Zhao S, Yuan H, Xie C, Dan X. D., J Chromatogr A. 1107 (2006) 290.

Wei S, Zhao F, Xu Z, Zeng B., Microchim Acta. 152 (2006) 285.

Mirmoghtadaie L. A, Ensafi A, Kadivar M, Norouzi P., Sci Eng. C 33 (2013) 1753.

Höller U, Brodhag C, Knöbel A, Peter Hofmann P, Spitzer V., J Pharm Biomed Anal. 31 (2003) 151.

Amidžić R, Brboric J, Čudina O, Vladimirov S., J Serb Chem Soc. 70 (2005) 1229.

Radhika K, Srinath N, Sumanth K. S, Neelima S, Krishna V.P., KIJAP 3 (2012) 701.

Nelson B.C. E, Sharpless K. E, Sander L.C. y., J Chromatogr A. 1135 (2006) 203.

Shishehbore M. R, Sheibani A, Haghdost A., Spectrochim Acta Part A. 81(2011) 304.

Ashour S, Khateeb M., Arabian J Chem. 4 (2011) 299.

Ashour S, Bahbouh M, Khateeb M., Int J Biomed Sci. 6 (2010) 19.

Kopanica M, Satra V, Echschlager K, Rorsak I, Koduys Z, Sandr K, Eds., Elsevier, Amsterdam, The Netherlands, (1983).

Persez-Bendit, D., Silva, M., chapter 11, John Wiley & Sons, New York, NY, USA. (1988).

Ringbom A, Zeitschrift für Analytische Chemie. 115 (1939) 332.

Morelli B., Analyst (London). 108 (1983) 870.

Nalimov V.V., Pergamon Press, Oxford. (1963) 189.

Morelli B., J Pharm Biomed Anal. 5 (1987) 577.

Morelli B., Analyst. 113 (1988) 1077.

Rose J., Advanced Physico-Chemical Experiments, Pittman, London, UK. (1964). ( Received 04 April 2015; accepted 24 April 2015 ).

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