Paper Titles in Periodical
International Letters of Chemistry, Physics and Astronomy
Volume 33

Subscribe

Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

ILCPA > Volume 33 > Simultaneous Determination of Aspirin and...
< Back to Volume

Simultaneous Determination of Aspirin and Rosuvastatin Calcium in Capsules by Using RP-HPLC Coupled with Photo Diode Array Detection

Full Text PDF

Abstract:

A simple, sensitive, specific, and cost effective method for simultaneous determination of Aspirin and Rosuvastatin calcium was developed and validated in single dosage formulation. The sample solution of ASP and RSTC was prepared using methanol as a solvent. Separation of ASP and RSTC was achieved with a mobile phase consisting of 20 mM KH2PO4 : Methanol (30:70 v/v) at a flow rate of 1.0 ml/min. Separations were performed on Merck hibar 250-4.6 RP18 (5 µm) column (150 mm X 3.0 mm), using a Shimadzu Prominence HPLC system equipped with a Shimadzu SPD-20A detector, Rhenodyne 7725i injector with 20 μL loop, LC-20 AD pump, CBM-20 Alite controller and LC Solution software. Retention times of ASP and RSTC were 3.747 and 5.969 minutes respectively. Absolute recovery of ASP and RSTC was 100.3 and 100.03 % respectively. The lower limit of quantification (LLOQ) of ASP and RSTC was 0.3097 and 0.1063 ppm and lower limit of detection (LLOD) of ASP and RSTC was 0.01535 and 0.01358 ppm respectively. Linearity was established for the range of concentrations 15.00-90.0 μg/ml and 2.0-12.0 μg/ml for ASP and RSTC respectively with the coefficient of determination (R2) of 0.994 and 0.999 for both the compounds. The inter- and intra-day precision in the measurement of ASP quality control (QC) sample 75 μg/ml, were in the range 0.1-0.2 % relative standard deviation (R.S.D.) and 0.2-0.3 % R.S.D., respectively. The inter- and intra-day precision in the measurement of RST quality control (QC) sample 10 μg/ml, were in the range 0.1-0.2 % R.S.D., and 0.0-0.3 % R.S.D., respectively. The developed method would be applicable for routine quality control of ASP And RSTC in bulk as well as in pharmaceutical formulations

Info:

Periodical:
International Letters of Chemistry, Physics and Astronomy (Volume 33)
Pages:
218-230
DOI:
https://doi.org/10.18052/www.scipress.com/ILCPA.33.218
Citation:
A. Panchal et al., "Simultaneous Determination of Aspirin and Rosuvastatin Calcium in Capsules by Using RP-HPLC Coupled with Photo Diode Array Detection", International Letters of Chemistry, Physics and Astronomy, Vol. 33, pp. 218-230, 2014
Online since:
May 2014
Authors:
Ankita Panchal, Gaurav Sanghvi, Ashish Vachhani, Navin Sheth, Devendra Vaishnav
Keywords:
Aspirin, Capsule, Rosuvastatin Calcium, RP-HPLC, Validation
Export:
RIS, BibTeX, Text
Distribution:
Open Access

Creative Commons License
This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

F. Catella-Lawson, M. P. Reilly, S. C. Kapoor, A. J. Cucchiara, S. DeMarco, B. Tournier.

S. N. Vyas, G. A. FitzGerald, N. Engl. J. Med. 345(25) (2001) 1809-1817.

C. D. Funk, L. B. Funk, M. E. Kennedy, A. S. Pong and G. A. Fitzgerald, FASEB J. 5(9) (1991) 2304-2312.

F. McTaggart, Atheroscler Suppl. 4(1) (2003) 9-14.

F. McTaggart, L. Buckett, R. Davidson, G. Holdgate, A. McCormick, D. Schneck, G. Smith, M. Warwick, Am. J. Cardiol. 87(5A) (2001) 28B-32B.

A. Karunakaran, V. Subhash, R. Chinthala and J. Muthuvijayan, Stamford J Pharm Sci. 4(1) (2011) 58-63.

Z. Kokot, K. Burda, J. Pharm. Biomed. Anal. 18(4-5) (1998) 871-875.

P. C. Schmidt, B. W. Glombitza, Trends Anal Chem 14(2) (1995) 45-49.

V. J. Ferreira, A. C. Cavalheiro, E. Fagnani, M. d. Moraes, L. Pezza, H. R. Pezza, C. B. Melios, Analyt Sci 15(3) (1999) 249-253.

M. S. Elmasry, I. S. Blagbrough, M. G. Rowan, H. M. Saleh, A. A. Kheir,P. J. Rogers, J. Pharm. Biomed. Anal. 54(4) (2011) 646-652.

M. Gandhimathi, T. K. Ravi, A. Abraham, R. Thomas, J. Pharm. Biomed. Anal. 32(6) (2003) 1145-1148.

F. Gaspari, M. Locatelli, Ther. Drug Monit. 9(2) (1987) 243-247.

Y. Shah, Z. Iqbal, L. Ahmad, A. Khan, M. I. Khan, S. Nazir, F. Nasir, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 879(9-10) (2011) 557-563.

L. H. Shaw, T. H. Tsai, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 895-896 (2012) 31-38.

R. Thomis, E. Roets, J. Hoogmartens, J. Pharm. Sci. 73(12) (1984) 1830-1833.

V. K. Bhusari, S. R. Dhaneshwar, ISRN Analyt Chem, 2012 (2012) Article ID 609706.

B. Chaudhari, N. Patel and P. Shah, Indian J. Pharm. Sci., 69(1) (2007) 130.

H. A. Raj, S. J. Rajput, J. B. Dave, C. N. Patel, Int J ChemTech Res 1(3) (2009) 677-689.

M. Sharma, S. Sharma, D. Kohli, A. Sharma, Arch Appl Sci Res 2(1) (2010) 1-7.

D. Vora, A. Kadav, J. Liq. Chromatogr. Related Technol. 31(18) (2008) 2821-2837.

C. K. Hull, P. D. Martin, M. J. Warwick, E. Thomas, J. Pharm. Biomed. Anal. 35(3) (2004) 609-614.

K. Lan, X. Jiang, Y. Li, L. Wang, J. Zhou, Q. Jiang, L. Ye, J. Pharm. Biomed. Anal. 44(2) (2007) 540-546.

R. K. Trivedi, R. R. Kallem, R. Mullangi, N. R. Srinivas, J. Pharm. Biomed. Anal. 39(3-4) (2005) 661-669.

J. Martin, W. Buchberger, E. Alonso, M. Himmelsbach, I. Aparicio, Talanta 85(1) (2011) 607-615.

G. B. Kasawar, M. Farooqui, J. Pharm. Biomed. Anal. 52(1) (2010) 19-29.

ICH, Q2(R1)(2005) 1-13. ( Received 03 May 2014; accepted 10 May 2014 ).

Show More Hide