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Validation of HPLC Method for Quantitative Determination of Pirimiphos Methyl

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Abstract:

The objective of this research to optimise the HPLC method was developed for quantitative determination of Pirimiphos-methyl. Chromatographic separation was achieved on a 250 x 4.6 mm i.d. reversed phase column Qualisil BDS 5u C18, Using deionized acetonitrile:water in the ratio of 85:15 v/v respectively as mobile phase. The eluent was monitored at 254 nm. A sharp peak was obtained for the Pirimiphos-methyl at 9.29 min. The UV Spectrophotometric method performed at 254 nm after full scan analysis using methanol as a solvent. The result revealed that both methods are suitable to carry out routine analysis of Pirimiphos methyl, However HPLC results showed high precise, accurate and sensitive than the UV Spectrophotometer. Hence HPLC method is suitable for trace analysis of Pirimiphos methyl in environmental samples.

Info:

Periodical:
International Letters of Chemistry, Physics and Astronomy (Volume 28)
Pages:
93-102
Citation:
C. Tamilselvan et al., "Validation of HPLC Method for Quantitative Determination of Pirimiphos Methyl", International Letters of Chemistry, Physics and Astronomy, Vol. 28, pp. 93-102, 2014
Online since:
Feb 2014
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References:

IE. Mills, Pirimiphos-methyl: blood concentrations and tissue retention in rat. Report No CTL/P/247. Imperial Chemical industries, Central toxicology laboratory, (1976)11.

C. Cox, Journal of Pesticide Reform 2 (1996) 2.

C. Sobarzo, E. Bustoos-Obregón, Asian Journal of Andrology 2 (2000) 147.

E. ustos-Obregón, P. Gonzállez-Hormanzábal, Int. J. Morphol. 2 (2003) 155.

A. E. Ghaly, F. Alkoaik, A. Snow, Canadian Biosystems Engineering 49 (2007) 61.

Hendig Winarno, Agustin N. M. Bagyo, Winarti A. Lindu, Ermin K. Winarno, Atom Indonesia 30(2) (2004) 35.

US Food and Drug Administration, Pesticide Analytical Manual, Vol. I, FDA, Rockville, MD, (1979).

P. Lopez-Roldan, M. J. Lopez de Alda, D. Barcelo, Anal. Bioanal. Chem. 378 (2004) 599.

A. Pasha, Y. N. Vijayashankar, N. G. K. Karanth, J. AOAC Int. 79 (1996) 1009.

A. Di Corcia, M. Marchetti, Anal. Chem. 63 (1991) 580.

O. A. Zalat, M. A. Elsayed, M. S. Fayed, M. K. Abd El Megid, International Letters of Chemistry, Physics and Astronomy 2 (2014) 58-63.

S. Magdic, A. Boyd-Boland, K. Jinno, J. B. Pawliszyn, J. Chromatogr. A 736 (1996) 219.

P. L. Wylie, K. Uchiyama, J. AOAC Int. 79 (1996) 571.

B. F. Scott, J. Struger, H. Tse, J. Environ. Anal. Chem. 61 (1995) 129.

N. K. Wilson, J. C. Chuang, C. Lyu, J. Expo. Anal. Environ. Epidemiol. 11 (2001) 449.

D. E. Glotfelty, M. S. Majewski, J. N. Seiber, Environ. Sci. Technol. 24 (1990) 353. ( Received 15 February 2014; accepted 19 February 2014 ).

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