Subscribe

Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

ILCPA > Volume 28 > Validation of HPLC Method for Quantitative...
< Back to Volume

Validation of HPLC Method for Quantitative Determination of Pirimiphos Methyl

Full Text PDF

Abstract:

The objective of this research to optimise the HPLC method was developed for quantitative determination of Pirimiphos-methyl. Chromatographic separation was achieved on a 250 x 4.6 mm i.d. reversed phase column Qualisil BDS 5u C18, Using deionized acetonitrile:water in the ratio of 85:15 v/v respectively as mobile phase. The eluent was monitored at 254 nm. A sharp peak was obtained for the Pirimiphos-methyl at 9.29 min. The UV Spectrophotometric method performed at 254 nm after full scan analysis using methanol as a solvent. The result revealed that both methods are suitable to carry out routine analysis of Pirimiphos methyl, However HPLC results showed high precise, accurate and sensitive than the UV Spectrophotometer. Hence HPLC method is suitable for trace analysis of Pirimiphos methyl in environmental samples.

Info:

Periodical:
International Letters of Chemistry, Physics and Astronomy (Volume 28)
Pages:
93-102
DOI:
10.18052/www.scipress.com/ILCPA.28.93
Citation:
C. Tamilselvan et al., "Validation of HPLC Method for Quantitative Determination of Pirimiphos Methyl", International Letters of Chemistry, Physics and Astronomy, Vol. 28, pp. 93-102, 2014
Online since:
Feb 2014
Export:
Distribution: