Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

ILCPA > ILCPA Volume 21 > Validation of UV Spectrophotometric and HPLC...
< Back to Volume

Validation of UV Spectrophotometric and HPLC Methods for Quantitative Determination of Chlorpyrifos

Full Text PDF


A specific and sensitive HPLC and UV spectrophotometric methodwere developed for determination and analysis of chlorpyrifos. Chromatographic separation was achieved on a 150 mm x 10 mm I.D. reversed phase column Zorbax SB C-18. usingdeionizedwater: acetonitrile in the ratio of 10:90 v/v respectively as mobile phase. The effluent was monitored at 290 and 230 nm. Two sharp peaks were obtained for the solvent and chlorpyrifos at 2.7 and 3.45 min respectively. UV spectrophotometric method was performed at 290 nm using Isopropanol as the solvent. Linear range was 0.025-3500 ppm (r2 = 0.9986 ±0.0009) for HPLC method and 2.229 to 200 ppm (r2 = 0.9988) for UV spectrophotometric method. Validation guidelines and statistical analysis showed that both the methods were precise, accurate, sensitive, and can be used for the routine quality control of chlorpyrifos in waste discharges


International Letters of Chemistry, Physics and Astronomy (Volume 21)
O.A. Zalat et al., "Validation of UV Spectrophotometric and HPLC Methods for Quantitative Determination of Chlorpyrifos", International Letters of Chemistry, Physics and Astronomy, Vol. 21, pp. 58-63, 2014
Online since:
November 2013

[1] Sultatos L. G., S. D. Murphy, Fund. Appl. Toxicol. 3 (1983) 16-21.

[2] Sultatos L. G., M. Shao, S. D. Murphy, Toxicol. Appl. Phramacol. 73 (1984) 60-68.

[3] The National Registration Authority (NRA), Review of Chlorpyrifos, Septmber 2000, Volume 1.

[4] A. Goldstein, L. Aronow, S. M. Kalman. Principles of Drug Action: The Basis of Pharmacology, Wiley, New York, (1974).

[5] D. Grob, J. C. Harvey, J. Clin. Invest. 37 (1958) 350.

[6] US Food and Drug Administration, Pesticide Analytical Manual, Vol. I, FDA, Rockville, MD, (1979).

[7] P. Lopez-Roldan, M. J. Lopez de Alda, D. Barcelo, Anal. Bioanal. Chem. 378 (2004) 599.

[8] A. Pasha, Y. N. Vijayashankar, N. G. K. Karanth, J. AOAC Int. 79 (1996) 1009.

[9] A. Di Corcia, M. Marchetti, Anal. Chem. 63 (1991) 580.

[10] S. Magdic, A. Boyd-Boland, K. Jinno, J. B. Pawliszyn, J. Chromatogr. A 736 (1996) 219.

[11] P. L. Wylie, K. Uchiyama, J. AOAC Int. 79 (1996) 571.

[12] B. F. Scott, J. Struger, H. Tse, J. Environ. Anal. Chem. 61 (1995) 129.

[13] N. K. Wilson, J. C. Chuang, C. Lyu, J. Expo. Anal. Environ. Epidemiol. 11 (2001) 449.

[14] D. E. Glotfelty, M. S. Majewski, J. N. Seiber, Environ. Sci. Technol. 24 (1990) 353. (Received 29 October 2013; accepted 03 November 2013).

Show More Hide
Cited By:
This article has no citations.