Subscribe

Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

IJPPE > IJPPE Volume 9 > Diuretic Activity of Hibiscus sabdariffa L. in...
< Back to Volume

Diuretic Activity of Hibiscus sabdariffa L. in Wistar Rats

Full Text PDF

Abstract:

The diuretic effect of aqueous extract of Hibiscus sabdariffa has always been noted by the early botanists who studied the plant. On the other hand, very little scientific work to confirm this state of affairs has been done. In this pharmacological study, we provide scientific evidence to previous observations. Using an electrolyte analyzer to evaluate urinary volume and ions and appropriate experimental protocols on wistar strain rats, we have shown that: The aqueous extract of Hibiscus sabdariffa (AEHS) administered at doses of 200, 500 and 700 mg / kg BW induces a dose-dependent and significant increase in urinary excretion in rats. This increase was 12.75 ± 1.971 mL / kg, 23.20 ± 2.082 mL / kg and 29.42 ± 2.097 mL / kg BW, respectively is after 24 hours. That is a respective increase of 55.5, 182 and 333% compared to the control. The aqueous extract of Hibiscus sabdariffa (AEHS) uses at the dose of 700 mg / kg BW results in a reduction of the first urination time by 26.30% compared to the control. The determination of electrolytes in rats treated with 700 mg / kg BW after 24 hours showed high concentrations of sodium, potassium and chlorine in the urine. These different concentrations are for sodium of 72.51%, potassium of 76.54% and 58.81% for chlorine relative to the concentration of the controls. Compared to AEHS, hydrochlorothiazide at 25 mg / kg BW results in urinary excretion of 80.79% sodium, 85.30% potassium and 75.97% chlorine. Similarly with furosemide uses at 20 mg / kg BW), the concentrations are 86% sodium, 79% potassium and 71.58% chlorine. Compared with control rats, the Na + / K + ratio did not vary significantly in the treated animals. AEHS is therefore a diuretic substance like hydrochlorothiazide and furosemide.

Info:

Periodical:
International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 9)
Pages:
10-17
Citation:
A. Mea et al., "Diuretic Activity of Hibiscus sabdariffa L. in Wistar Rats", International Journal of Pharmacology, Phytochemistry and Ethnomedicine, Vol. 9, pp. 10-17, 2018
Online since:
April 2018
Export:
Distribution:
References:

[1] L. Amar et al., Aldosterone synthase inhibition with LCI699: a proof-of-concept study in patients with primary aldosteronism, Hypertension. 56(5) (2010) 831-838.

[2] A.K. Amonkan et al., Comparative effects of different fractions of crude aqueous extract of Ficus exasperata leaves on blood pressure, International Current Pharmaceutical Journal. 2(11) (2013) 193-195.

[3] M.J. Bia, R.A. DeFronzo, Extrarenal potassium homeostasis, Am. J. Physiol. 240(4) (1981) F257–F268.

[4] C.C. Chen et al., Hibiscus sabdariffa extract inhibits the development of atherosclerosis in cholesterol-fed rabbits, Journal of Agricultural and Food Chemistry. 51(18) (2003) 5472-5477.

[5] B.A. Di Tollo, Aspects nutritionnels des cardiopathies chez le chat, The National Academies Press, Washington D.C., 2006, pp.112-120.

[6] K.M. Gaafa, M.M. Badawy, A.A Hamza, The protective effects of ascorbic acid, cimetidine, and nifedipine on diethyldithiocarbamate-induced hepatic toxicity in albino rats, Drug Chem Toxicol. 34(4) (2011) 405-419.

[7] J. Kerharo, J.G. Adam, La pharmacopée sénégalaise traditionnelle, plantes médicales et toxiques, Ed. Vigot frères, Paris, (1974).

[8] K.H. Kraus, The use of desmopressin in diagnosis and treatment of diabetes insipidus in cats, Compend. Cont. Educ. Pract. Vet. 9 (1987) 752-756.

[9] C.L. Liu et al., In vivo protective effect of protocatechuic acid on tert-butyl hydroperoxide-induced rat hepatotoxicity, Food and Chemical Toxicology. 40(5) (2002) 635-641.

[10] A. Mea et al., Investigation and mechanisms of action of the aqueous extract of Hibiscus sabdariffa (AEHS) on the aorta isolated from guinea pig and on blood pressure of rabbit, Asian Journal of Science and Technology. 07(11) (2016) 3932-3938.

[11] P. Meier, M. Burnier, Place des diurétiques de l'anse dans le traitement de l'hypertension artérielle, Rev. Med. Suisse. 2 (2002).

[12] B. Odlind, Site and mechanism of the action of diuretics, Basic & Clinical Pharmacology & Toxicology. 54(s1) (1983) 5-15.

[13] M.D. Okusa et al., Active potassium absorption by the renal distal tubule, Am. J. Physiol. 262 (1992) F488–93.

[14] P.E. Joshua et al., Hepatocurative effect of aqueous extract of Hibiscus sabdariffaon some antioxidants and haematological indices of acetaminophen-challenged Wistar albino rats, African Journal of Pharmacy and Pharmacology. 11(20) (2017).

[15] J.A. Payne, B. Forbush, Alternatively spliced isoforms of the putative renal Na-K-Clcotransporter are differentially distributed within the rabbit kidney, Proceedings of the National Academy of Sciences. 91(10) (1994) 4544-4548.

[16] J.L. Pousset, Plantes médicinales africaines. Utilisation pratique, Agence de coopération culturelle et technique (ACCT), (1989).

[17] J.M. Russell, Sodium-potassium-chloride cotransport, Physiol. Rev. 80(1) (2000) 211-276.

[18] S. Silbernagl, A. Despopoulos, Taschenatlas der Physiologie, Thieme Verlag, Stuttgart, (2003).

Show More Hide
Cited By:
This article has no citations.