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Investigations of Sedative and Antidiarrheal Activity of Commelina appendiculata in Swiss Albino Mice

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The aim of the present study was to investigate the sedative and antidiarrheal activity of ethanol extract of C. appendiculata at the doses of 100 and 200 mg/kg body weight per oral (p.o) in Swiss albino mice. Sedative activity of the extract (100 and 200 mg/kg, p.o.) was assessed utilizing thiopental sodium-induced sleeping time model to evaluate sedative potential of the extract, hole cross to evaluate motor activity and open field test to evaluate exploratory behaviour. The extract also produced rapid onset and maximized the duration of sleeping time when administered with thiopental sodium. The extract fundamentally (p < 0.05-0.001) diminished motor action and exploratory conduct of mice in hole cross and open field test separately. In antidiarreal activity study, the concentrate (200 mg/kg) likewise demonstrated most extreme inhibition of defecation (90.64% and 80.95%) in both models individually. The present study demonstrates that the ethanol extract of C. appendiculata have central nervous system (CNS) depressant and antidiarrheal properties. In this manner, the plant may be further investigated to find for its pharmacological active natural products.


International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 7)
P. R. Dash et al., "Investigations of Sedative and Antidiarrheal Activity of Commelina appendiculata in Swiss Albino Mice", International Journal of Pharmacology, Phytochemistry and Ethnomedicine, Vol. 7, pp. 9-17, 2017
Online since:
Jun 2017

[1] K.U. Siddique et al., Encyclopedia of flora and fauna of Bangladesh. Angiosperms: Monocotyledons (Agavaceae-Najadaceae), Asiatic Society of Bangladesh, Dhaka. 11 (2007) 145.

[2] P.G. Xiao, New edited records of Traditional Chinese Medicine, Chemical Industry Press, Beijing, vol. 3, (2003).

[3] X.Y. Tang, M.H. Zhou, Y.B. Zhang, Active constituents of Commelina communis L, China Journal of Chinese Materia Medica. 19(5) (1994) 297-298.

[4] O.K. Kim et al., Screening of edible Japanese plants for nitric oxide generation inhibitory activities in RAW 264. 7 cells, Cancer Letters. 125 (1998) 199-207.

[5] P.R. Dash, M.S. Rana, M.S. Ali, Investigation of analgesic and cytotoxic activities of ethanol extract of Commelina appendiculata, J. Pharmacogn. Phytochem. 4(3) (2015) 53-59.

[6] S.C. Sharma, T J.S. Andon, A dammarane triterpene from Commelina undulata, Phytochemistry. 21 (1982) 2420-2421.

[7] J.Z. Stirton, J.B. Harborne, Two distinctive anthocyanin patterns in the commelinaceae, Biochem. Syst. Ecol. 8(3) (1980) 285-287.

[8] Botanical Institute of Jiangsu Province, Compendium of Xinhua Materia Medica, 2nd ed., Vol. II. Scientific and Technological Press of Shanghai, 1991, p.574.

[9] M. Shiono, N. Matsugaki, K. Takeda, Structure of commelinin, a blue complex pigment from the blue flowers of Commelina communis, Proceedings Japan Acad. Series B. 84(10) (2008) 52-56.

DOI: 10.2183/pjab.84.452

[10] T. Goto et al., The real anthocyanin present in blue-colored flower petals of Commelina communis, Tetrahedron Lett. 24 (1983) 4863-4866.

DOI: 10.1016/s0040-4039(00)94027-9

[11] T. Hondo et al., Structural basis of blue-colour development in flower petals from Commelina communis, Nature. 358 (1992) 515-518.

DOI: 10.1038/358515a0

[12] X.Y. Tang, M.H. Zhou, Y.B. Zhang, Active constituents of Commelina communis L., China J. Chin. Mater. Med. 19 (1994) 297-298.

[13] Q. Yang, G. Ye, A new C-glucoside from Commelina communis, Chem. Nat. Comp. 45 (2009) 59-60.

[14] Q. Yang, G. Ye, W.M. Zhao, Chemical constituents of Commelina communis Linn, Biochem. Syst. Ecol. 35(9) (2007) 621-623.

[15] M.S. Ali, P.R. Dash, M. Nasrin, Study of sedative activity of different extracts of Kaempferia galangal in Swiss albino mice, BMC Complement Altern. Med. 15 (2015) 158.

[16] C.I. Walker et al., Antinociceptive activity of Mirabilis jalapa in mice, J. Ethnopharmacol. 120 (2008) 169–175.

[17] R.A. Turner, Screening methods in pharmacology, New York and London, Academic Press, 1965, p.64–69.

[18] K. Takagi, M. Watanabe, H. Saito, Studies on the spontaneous movement of animals by the hole cross test: Effect of 2-dimethylaminoethan its acylesters on the central nervous system, The Japanese Journal of Pharmacology. 21(6) (1971) 797-810.

[19] B.D. Gupta, P.C. Dandiya, M.L. Gupta, A psychopharmacological analysis of behaviour in rat, The Japanese Journal of Pharmacology. 21(3) (1971) 293-298.

[20] S.F.G. Hoba, M. Thomas, Study of antidiarrhoeal activity of four medicinal plants in castor-oil induced diarrhea, J. Ethnopharmacol. 76 (2001) 73-76.

DOI: 10.1016/s0378-8741(00)00379-2

[21] N.S. Doherty, Inhibition of arachidonic acid release as the mechanism by which glucocorticoids inhibit endotoxin-induced diarrhea, Br. J. Pharmacol. 73 (1981) 549–554.

[22] M.C. Lu, Studies on the sedative effects of Cistanche deserticola, J. Ethnopharmacol. 59 (1998) 161–165.

[23] K. Gamaniel et al., Pharmacological profile of NIPRD 94/002/1 – 0. A novel herbal antisickling agent, J. Pharmaceut. Res. Dev. 3(2) (1998) 89–94.

[24] R.M.G. Perez et al., Neuropharmacological activity of Solanum nigrum fruit, J. Ethnopharmacol. 62 (1998) 43–48.

[25] A. Verma et al., Pharmacological evaluation of Saraca indica Leaves for central nervous system depressant activity in mice, J. Pharm. Sci. Res. 2(6) (2010) 338-343.

[26] O.T. Kolawole, J.M. Makinde, O.A. Olajide, Central nervous depressant activity of Russelia equisetiformis, Niger. J. Physiol. Sci. 22(1-2) (2007) 59-63.

[27] S.K. Bhattacharya, K.S. Satyan, Experimental methods for evaluation of psychotropic agents in rodents: I-Anti-anxiety agents, Indian J. Exp. Biol. 35 (1997) 565-575.

[28] R.N. Takahashi, T.C. de Lima, G.S. Morato, Pharmacological action of tannic acid; ІІ. Evaluation of CNS activity in animals, Planta Med. 52 (1986) 272-275.

[29] E. Beubler, H. Juan, Effect of ricinoleic acid and other laxatives on net water flux and prostaglandin E release by the rat colon, J. Pharm. Pharmacol. 31 (1979) 681–685.

[30] T.S. Gaginella, S.F. Phillips, Ricinoleic acid: Current view of an ancient oil, Am. J. Dig. Dis. 20 (1975) 1171–1177.

DOI: 10.1007/bf01070759

[31] N. Mascolo et al., Inhibitors of nitric oxide synthetase prevent castor-oil-induced diarrhoea in the rat, Br. J. Pharmacol. 108 (1993) 861–864.

[32] J. Gálvez et al., Antidiarrhoeic activity of Sclerocarya birrea bark extract and its active tannin constituent in rats, Phytother. Res. 5 (1991) 276–278.

[33] S.J. Uddin et al., Antidiarrhoeal activity of the methanol extract of the barks of Xylocarpus moluccensis in castor oil- and magnesium sulphateinduced diarrhoea models in mice, J. Ethnopharmacol. 101 (2005) 139–143.

[34] P.K. Mukherjee et al., Antidiarrhoeal evaluation of Nelumbo nucifera rhizome extract, Indian J. Pharmacol. 27 (1995) 262–264.

[35] A. Agunu et al., Evaluation of five medicinal plants used in diarrhoea treatment in Nigeria, J. Ethnopharmacol. 101 (2005) 27–30.

[36] S. Mandal et al., Antidiarrhoeal activity of carbazole alkaloids from Murraya koenigii Spreng (Rutaceae) seeds, Fitoterapia. 81 (2010) 72–74.

[37] T.A. Abere, P.E. Okoto, F.O. Agoreyo, Antidiarrhea and toxicological evaluation of the leaf extract of Dissotis rotundifolia Triana (Melastomataceae), BMC Complement Altern. Med. 10(1) (2010) 71.

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