Phytochemical Profile and Antifungal Activity of Leaves Methanol Extract from the Psydrax dicoccos (Gaertn) Teys. &amp; Binn. Rubiaceae Family

Umaiyambigai, D.; Saravanakumar, K.; Adaikala Raj, G.

doi:10.18052/www.scipress.com/IJPPE.7.53

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Phytochemical Profile and Antifungal Activity of Leaves Methanol Extract from the Psydrax dicoccos (Gaertn) Teys. & Binn. Rubiaceae Family

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Abstract:

The present study was aimed to antifungal activity and phytoconstituents of leaves from the Psydrax dicoccos Gaertn. The antifungal activity of methanol extract from the P. dicoccos against Candida albicans, C. krusei, C. guilliermondii, C. parapsilosis, C. tropicalis, C. glabrata, four dermatophytes viz., Trichophyton rubrum, T. mentagrophytes, Microsporum gypseum and Epidermophyton flocossum. The methanol extract of the leaves were subjected to Fourier transform infrared spectroscopy (FT-IR) and Gas Chromatography-Mass Spectroscopic (GC-MS) analysis. The mean zones of inhibition produced by the tested extract in disc diffusion assays against fungal strains were ranged from 7.3 to 15.5 mm. The MIC values were between 125 and 500 µg/ml while, the MFC values were between 250 and 1000 µg/ml. The highest mean zones of inhibition (15.5±0.6 mm) was observed with methanol extract of P. dicoccos against C. albicans. The GC-MS analysis of P. dicoccos leaves showed the presence of cinnamic acid, 2H-1-Benzopyran-2-one,5,7-dimethoxy,(Z)6,(Z)9-Pentadecadien-1-ol, Benzofuran and n-Hexadecanoic acid as major compounds. Finally it can be concluded that the antifungal activity may be present in cinnamic acid, 2H-1-Benzopyran-2-one, 5, 7-, (Z) 6,(Z)9-Pentadecadien-1-ol, n-Hexadecanoic acid from the methanol extract from the P. dicoccos is highly valuable in medicinal usage and have fewer side effects.

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Periodical:

International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 7)

Pages:

53-61

DOI:

https://doi.org/10.18052/www.scipress.com/IJPPE.7.53

Citation:

D. Umaiyambigai et al., "Phytochemical Profile and Antifungal Activity of Leaves Methanol Extract from the Psydrax dicoccos (Gaertn) Teys. & Binn. Rubiaceae Family", International Journal of Pharmacology, Phytochemistry and Ethnomedicine, Vol. 7, pp. 53-61, 2017

Online since:

June 2017

Authors:

D. Umaiyambigai, K. Saravanakumar, G. Adaikala Raj

Keywords:

FT-IR, GC-MS, Phytochemical Profile, Psydrax dicoccos

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Open Access

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Creative Commons Attribution 4.0 International License

References:

[1] T.J. Walsh, A.H. Groll, Emerging fungal pathogens: evolving challenges to immunocompromised patients for the twenty-first century, Transplant Infect. Dis. 1(4) (1999) 247–261.

DOI: https://doi.org/10.1034/j.1399-3062.1999.010404.x

[2] R.V. Fleming, T.J. Walsh, E.J. Anaissie, Emerging and less common fungal pathogens, Infect Dis. Clin. North Am. 16 (2002) 915–933.

DOI: https://doi.org/10.1016/s0891-5520(02)00041-7

[3] V.K. Grover, R. Babu, S.P.S. Bedi, Steroid therapy current indications in practice, Ind. J. Anaesthesia. 51(5) (2007) 389-393.

[4] E. Pinto et al., Antifungal activity of the essential oil of Thymus pulegioides on Candida, Aspergillus and dermatophyte species, J. Med. Microbiol. 5 (2006) 1367–1373.

DOI: https://doi.org/10.1099/jmm.0.46443-0

[5] Z. Zhang et al., Natural products inhibiting Candida albicans secreted aspartic proteases from Tovomita krukovii, Planta Med. 68(1) (2002) 49–54.

DOI: https://doi.org/10.1055/s-2002-20049

[6] V. Krcmery, A.J. Barnes, Non-albicans Candida spp. causing fungaemia: pathogenicity and antifungal resistance, J. Hosp. Infect. 50(4) (2002) 243–260.

DOI: https://doi.org/10.1053/jhin.2001.1151

[7] C.J. Jessup et al., Antifungal susceptibility testing of dermatophytes: establishing a medium for inducing conidial growth and evaluation of susceptibility of clinical isolates, J. Clin. Microbiol. 38(1) (2003) 41-44.

[8] I. Weitzman, R.C. Summerbell, The dermatophytes, Clin. Microbiol. Rev. 8(2) (1995) 240–259.

[9] H.O. Edoga, D.E. Okwu, B.O. Mbaebie, Phytochemicals constituents of some Nigerian medicinal plants, Afr. J. Biotechnol. 4(7) (2005) 685-688.

[10] J. Mann, Secondary metabolism, Oxford University Press, London, (1978).

[11] K. Vasu et al., Biomolecular and phytochemical analyses of three aquatic angiosperms, Afr. J. Microbiol. Res. 3(8) (2009) 418-421.

[12] M.M. Cowan, Plant products as antimicrobial agents, Clin. Microbiol. Rev. 12(4) (1999) 564-582.

[13] G.M. Criagg, J.N. David, Natural product drug discovery in the next millennium, J. Pharm. Biol. 39 (2001) 8-17.

[14] F. Mojab et al., Phytochemicals screening of some species of Iranian plants, Iran J. Pharm. Res. 3 (2003) 77-82.

[15] J. Parekh, S. Chanda, Antibacterial and phytochemical studies on twelve species of Indian medicinal plants, Afr. J. Biomed. Res. 10 (2007) 175-181.

DOI: https://doi.org/10.4314/ajbr.v10i2.50624

[16] J. Parekh, S. Chanda, Phytochemicals screening of some plants from western region of India, Plant Arch. 8(2) (2008) 657-662.

[17] J.S. Karunyal, B. Andrews, Traditional medicinal plant wealth of Pachalur and Periyur hamlets, Dindigul district, Tamilnadu, Ind. J. Trad. Knowl. 9(2) (2010) 264-27.

[18] A.W. Bauer et al., Antibiotic susceptibility testing by a standardized single disc method, Am. J. Clin. Pathol. 45 (1966) 493-496.

[19] Clinical and Laboratory Standards Institute, Reference method for broth dilution antifungal susceptibility testing of yeasts; approved standard, third ed. CLSI Document M27-A3. Clinical Laboratory Standards Institute, Wayne, PA, USA (2008).

DOI: https://doi.org/10.1007/978-1-59745-134-5_2

[20] Clinical and Laboratory Standards Institute. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard, second ed. CLSI Document M38-A2. Clinical and Laboratory Standards Institute, Wayne, PA, USA (2008).

DOI: https://doi.org/10.1007/978-1-59745-134-5_2

[21] R.P. Adams, Identification of essential oil compounds by gas chromatography and mass spectrometry; 4th ed., Allured publishing Corporation, Coral Stream, IL (2009).

[22] D. Umaiyambigai, K. Saravanakumar, G.A. Raj, Phytochemical profiles, antibacterial and antifungal activity of leaves from the Psydrax dicoccos (Gaertn), Indo–Asian J. Multidisci. Res. 1(5) (2015) 422–431.

[23] F. Aquil, I. Ahmed, Broad spectrum of antibacterial and antifungal properties of certain traditionally used Indian medicinal plants, World J. Microbiol. Biotechnol. 19 (2003) 653-657.

[24] E.K. Barbour et al., Screening of selected indigenous plants of Lebanon for antimicrobial activity, J. Ethanopharmacol. 93 (2004) 1-7.

[25] R. Naz, A. Bano, Phytochemical screening, antioxidants and antimicrobial potential of Lantana camara in different solvents, Asian Pac. J. Trop. Dis. 3 (2013) 480-486.

DOI: https://doi.org/10.1016/s2222-1808(13)60104-8

[26] J. Ramirez et al., Antifungal activity of raw extract and flavanons isolated from Piper ecuadorense from Ecuador, Revista Brasileira de Farmacognosia. 23(2) (2013) 370-373.

DOI: https://doi.org/10.1590/s0102-695x2013005000012

[27] K. Rashed et al., Antibacterial and antifungal activities of methanol extract and phenolic compounds from Diospyros virginiana L., Industrial Crops and Products. 59 (2014) 210–215.

DOI: https://doi.org/10.1016/j.indcrop.2014.05.021

[28] I.L. Camacho-Hernandez et al., Antifungal activity of fruit pulp extract from Bromelia pinguin, Fitoterapia. 73(5) (2002) 411-413.

DOI: https://doi.org/10.1016/s0367-326x(02)00128-4

[29] K. Morteza-Semnani et al., Antifungal activity of the methanolic extract and alkaloids of Glaucium oxylobum, Fitoterapia. 74(5) (2003) 493-496.

DOI: https://doi.org/10.1016/s0367-326x(03)00113-8

[30] M. Chandrasekaran, V. Venkatesalu, Antibacterial and antifungal activity of Syzygium jambolanum seeds, J. Ethnopharmacol. 91 (2004) 105-108.

DOI: https://doi.org/10.1016/j.jep.2003.12.012

[31] N. Jaya Sree et al., Study on antimicrobial activity and chemical profiling of Pasburus missionis (Wight) Swingle, Inter. J. Pharm. Pharmaceut. Res. 2 (2015) 118-129.

[32] R. Venkatesh, R. Vidya, K. Kalaivani, Gas chromatography and mass spectrometry analysis of Solanum villosum (Mill. ) (Solanaceae), Inter. J. Pharma. Sci. Res. 5 (2014) 5283-5287.

[33] N.R. Rajeswari, S. Ramalakshmi, K. Muthuchelian, GC-MS Analysis of bioactive components from the ethanolic leaf extract of Canthium dicoccum (Gaertn. ) Teijsm & Binn, J. Chem. Pharma. Res. 3 (2011) 792-798.

[34] A. Rauf et al., Pistagremic acid, a novel antimicrobial and antioxidant isolated from Pistacia integerrima, Chem. Nat. Comp. 50 (2014) 97-99.

DOI: https://doi.org/10.1007/s10600-014-0875-2

[35] S.R. Suseem, M.A. Saral, Analysis on essential fatty acid esters of mushroom Pleurotus eous and its antibacterial activity, Asian. J. Pharma. Clin. Res. 6 (2013) 188-191.

[36] J. Coates, Interpretation of infrared spectra, a practical approach, in: Encyclopedia of analytical chemistry, R.A. Meyers (Ed. ), John Wiley & Sons Ltd, Chichester, 2000; pp.1085-10837.

[37] P. Ragavendran et al., Functional group analysis of various extracts of Aerva lanata (L., ) by FT-IR Spectrum, Pharmacologyonline. 1 (2011) 358-364.

[38] S. Mehrabian, A. Majd, I. Majd, Antimicrobial effects of three plants (Rubiatinctonum sp., Carthamus tinctorius and Juglans regia) on some airborne microorganisms, Aerbiol. 16 (2000) 455-458.

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