Contribution to the Ethnobotanical, Phytochemical, Antimicrobial and Antioxidant Study of the Leaves’ Aqueous Extract of the Common Walnut "Juglans regia L."

Bennacer, Amel; Cherif, Hamida Saida

doi:10.18052/www.scipress.com/IJPPE.7.41

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Contribution to the Ethnobotanical, Phytochemical, Antimicrobial and Antioxidant Study of the Leaves’ Aqueous Extract of the Common Walnut "Juglans regia L."

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Abstract:

This work has focused on phytochemical screening of the Common Walnut Juglans regia L. leaves, and biological tests: antioxidant and antimicrobial effects of the aqueous extracts (infusion, crude) of the common walnuts leaves that we’ve picked, dried and preserved. An ethnobotanical survey in Blida region has been achieved, where it has been concluded that the inhabitants of this region use the common walnuts leaves in their daily care, and the various organs of the Common walnut are widely used as well. The phytochemical screening based on specific tests helped to highlight the presence of the main secondary metabolites such as tannins, flavonoids, alkaloids, free quinones, anthocyanins and saponins, which could be responsible for the biological properties. The antimicrobial study was conducted on bacterial strains and a yeast showed that both aqueous (infusion, crude) extracts have antibacterial effect against certain bacterial strains: Staphylococcus aureus (40.43 mm), Staphylococcus epidermidis (35.36 mm), Sarcina lutea (31, 17 mm), respectively, and an inhibitory effect only for the crude aqueous extract of Eschirichia coli (12 mm), Klebsiella pneumoniae (12 mm), for against these two extracts were proved to be inactive for Candida albicans. In addition, the study of antioxidant activity by the method of reduction of iron (FRAP) and the method of reduction of free radical (DPPH) has revealed an average rate of aqueous extract (infusion) compared with that of ascorbic acid and rutin respectively. With an EC50 of the aqueous extract and rutin which equal to 0.18 and 0.017 mg/ml respectively. According to these results we confirm some information obtained during the ethnobotanical survey and that Juglans regia L. is endowed with an antimicrobial and antioxidant power.

Info:

Periodical:

International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 7)

Pages:

41-52

DOI:

https://doi.org/10.18052/www.scipress.com/IJPPE.7.41

Citation:

A. Bennacer and H. S. Cherif, "Contribution to the Ethnobotanical, Phytochemical, Antimicrobial and Antioxidant Study of the Leaves’ Aqueous Extract of the Common Walnut "Juglans regia L."", International Journal of Pharmacology, Phytochemistry and Ethnomedicine, Vol. 7, pp. 41-52, 2017

Online since:

June 2017

Authors:

Amel Bennacer, Hamida Saida Cherif

Keywords:

Antimicrobial, Antioxidant, Aqueous Extract (Infusion, Crude), Ethnobotanical Survey, Juglans regia L.

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Distribution:

Open Access

This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

[1] P. Iserin et al., Encyclopedia of Medicinal Plants, Larousse Edition, (2001).

[2] El-Rhaffari L., A. Zaid, Practice of phytotherapy in the south-east of Morocco (Tafilalet). An empirical knowledge for a renovated pharmacopoeia. Origin of traditional pharmacopoeias and elaboration of learned pharmacopoeias, 2004, pp.293-318.

[3] N. Hamza, Preventive effect of three medicinal plants used in the Wilaya of Constantine for the treatment of experimental type 2 diabetes induced by the diet-high fat- in C57BL / 6J mice, Doctorat Thesis, Science alimentaire. Constantine, 2011, p.16.

[4] L. Ali-Delille, The medicinal plants of Algeria, Berti Edition, Algiers, Algeria, (2010).

[5] T. Fukuda, H. Ito, T. Yoshida, Anitioxidative polyphenols from walnuts (Juglans regia L. ), Journal of Phytochemistry. 63 (2003) 795-801.

DOI: https://doi.org/10.1016/s0031-9422(03)00333-9

[6] J. Liu, Simultaneous determination of three diarylheptanoid and an alfa-tetralone derivative in the green walnut husks (Juglans regia L. ) by high performance liquid chromatography with photodiode array detector, Journal of Chromatography A. 1190(1) (2008).

DOI: https://doi.org/10.1016/j.chroma.2008.02.083

[7] M. Carvahlo et al., Human cancer cell antiproliferative and antioxidant activities of Juglans regia L., Journal of Food and Chemical Toxicology. (48) (2010) 441-447.

[8] H. Hosseinzadeh, H. Zarer, E. Taghiabadi, Antinociceptive, anti-inflammatory and acute toxicity effects of Juglans regia Leaves in mice, Iranian Red Crescent Medical Journal. 13(1) (2011) 27-33.

[9] M. Dupérat, J.M. Polese, Visual Encyclopedia of Trees and Shrubs, Artemis Edition France, (2008).

[10] B. Arnal et al., Phytotherapy: health through medicinal plants, Vidal Edition, Selection of Reader's Digest, Canada. 447 (2009) 167.

[11] F. Paris, P. Schawenberg, Guide to medicinal plants (3rd edition), (1977).

[12] J. Bruneton, Pharmacognosy: element of phytochemistry and pharmacognosy, 3rd edition, TEC and DOC, Paris, (1999).

[13] W T. Simpson, Drying and control of moisture content and dimensional changes, Edition: Forest Product Laboratory, Madison, (1999).

[14] W. Zerrad et al., Comparative study of the biochemical and molecular mechanisms of resistance to water stress of two varieties of hard wheat, Laboratory of Biochemistry, Environment and Agroalimentary, Morocco, (2006).

[15] V.G. De Billerbeck et al., Antibacterial and antifungal activity of products based on essential oils, Hygienes. 10(3) (2002) 248-251.

[16] C. Mutai et al., Antimicrobial activity of Acacia mellifera extracts and lupane triterpenes, Journal of Ethnopharmacology. 123(1) (2009) 143-148.

DOI: https://doi.org/10.1016/j.jep.2009.02.007

[17] N. Balasundram et al., Antioxydant properties of palm fruits extracts, Asia Pac. J. Clin. Nutr. 14(4) (2005) 319-324.

[18] M. Cuendet et al., Iridoid glucosides with three radical scarvenging properties from Fagraea blumei, Helvetica Chimica Acta. 80(4) (1997) 1144-1152.

DOI: https://doi.org/10.1002/hlca.19970800411

[19] M. Bruits, F. Bucar, Antioxydant activity of Nigella sativa essential oil, Phytother. Res. 14(5) (2000) 323-328.

DOI: https://doi.org/10.1002/1099-1573(200008)14:5<323::aid-ptr621>3.0.co;2-q

[20] R. Torres et al., Antioxidant activity of coumarins and flavonols from the resinous excudate of Haplopoppus multifolius, Photochemistry. 67(10) (2006) 984-987.

DOI: https://doi.org/10.1016/j.phytochem.2006.03.016

[21] M. Idolo, R. Motti, S. Mazzoleni, Ethnobotanical and phytochemical knowledge in a long-history protected area the Abruzzo, Lazio and Molise National park (Italian Apennines), Journal of Ethnopharmacology. 127(2) (2010) 379-395.

DOI: https://doi.org/10.1016/j.jep.2009.10.027

[22] V. Tene et al., An ethnobotanical survey of medicinal plants used in Loja and Zamora-Chinchipe, Ecuador, Journal of Ethnopharmacology. 111(1) (2007) 63-81.

DOI: https://doi.org/10.1016/j.jep.2006.10.032

[23] Z. Sher, Z. Khan, F. Hussain, Ethnobotanical studies of some plants of Chagharzai valley, discrit Buner, Pakistan, Pak. J. Bot. 43(3) (2011) 1445-1452.

[24] ANSM, Common Walnut for homeopathic preparations, French Pharmacopoeia, (2005).

[25] T.I. Shah, N. Ganesh, S. Akthar, Preliminary phytochemical evaluation and antibacterial potential of different leaf extracts of Juglans Regia: a uquibitous dry fruits from Kashmir India, Int. J. Pharm. Sci. Rev. Res. 18 (2013) 93-96.

[26] T. Nirmladevi et al., Pharmacognostic and phytochemical investigation of Juglans regia Linn. bark, Pharmacognosy Journal. 3(25) (2011) 39-43.

DOI: https://doi.org/10.5530/pj.2011.25.7

[27] J.L. Faucher, J.L. Avril, General and medical bacteriology, Volume 1, Ellipses (Ed), Paris, (2002).

[28] M A. Rather et al., Chemical composition, antioxidant and antibacterial activities of the leaf essential oil of Juglans regia L. and its constituents, Journal of Phytomedicine. 19 (2012) 1185-1190.

DOI: https://doi.org/10.1016/j.phymed.2012.07.018

[29] I. Oliveira et al., Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L. ) green husks, Journal of Food and Chemical Toxicology. (46) (2008) 2326-2331.

DOI: https://doi.org/10.1016/j.fct.2008.03.017

[30] P. Sharma et al., In vitro antibacterial and free radical scavenging activity of green hull of Juglans regia, Journal of Pharmaceutical Analysis. 3(4) (2013) 298-302.

DOI: https://doi.org/10.1016/j.jpha.2013.01.006

[31] I.F.F. Benzie, J.J. Strain, The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay, Analytical Biochemistry. 239(1) (1996) 70-76.

DOI: https://doi.org/10.1006/abio.1996.0292

[32] Y.C. Chung et al., Antioxidative activity and safety of the 50% ethanolic extrat from red bean fermented by Bacillus subtilis IMR-NK1, Journal of Agricultural and Food Chemistry. 50 (2002) 2454-2458.

DOI: https://doi.org/10.1021/jf011369q

[33] B.M. Silva et al., Quince (Cydonia oblonga Miller) fruit (Pulp, peel and seed) and jam: antioxidant activity, Journal of Agricultural and Food Chemistry. 52(15) (2004) 4705-4712.

DOI: https://doi.org/10.1021/jf040057v

[34] H. Ahmad, I. Khan, A. Wahid, Antiglycation and antioxidation properties of juglans regia and calendula officinalis: possible role in reducing diabetic complications and slowing down ageing, Journal of Traditional Chinese Medicine. 32(3) (2012).

DOI: https://doi.org/10.1016/s0254-6272(13)60047-3

[35] I.F. Almeida et al., Walnut (Juglans regia) leaf extracts are strong scavengers of pro-oxidant reactive species, Journal of Food Chemistry. 106(3) (2007) 1014-1020.

DOI: https://doi.org/10.1016/j.foodchem.2007.07.017

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