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Behavior Effect of Fluoxetine in Presence of Selenium Using Albino Mice

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Depression is a major cause of morbidity worldwide. Fluoxetine is a selective serotonin reuptake inhibitor, and is effective antidepressant medication. Selenium is essential for good health but required only in small amounts.Aim of the study is to investigate the effects of fluxetine alone and in presence of selenium on anxiety, spontaneous motor activity and antidepressant behavior. Also, the study aims to investigate the effects of selenium on spontaneous motor activity, anxiety measure, and antidepressant behavior, using photoelectric cells, elevated plus maze and forced swimming maze.Mice were divided into 5 group (n=6). Group 1 (control), administered 1% tween 80 (5 ml/kg); group 2 administered selenium (200 µg/kg); group 3 received diazepam as a positive control (1 mg/kg); group 4 received fluoxetine (20 mg/kg); while group 5 received combined treatment of fluoxetine and selenium. All drugs injected sub acutely (three doses), mice were intraperitoneally administered at 24, 5, and 1.0 hrs before scoring. All drugs administered as suspension in 1% Tween 80 (T80). It was injected in volume 5ml/kg. Plus maze, photoelectric cells and forced swimming maze models were used.Fluoxetine has no effect on anxiety or locomotor activity; while selenium produced anxiolytic effect without changes on locomotor activity. Fluoxetine has antidepressant activity without any effect on duration of climbing. Selenium induced antidepressant effect with climbing action. Fluoxetine abolish the anxiolytic effect of selenium when administered together, but the combined treatment decreases the locomotor activity. Fluoxetine administration with selenium counteract the antidepressant effect of each other and climbing effect of selenium. Finally, selenium improves anxiety and depression behavior in albino mice, and might be used as an alternative therapy instead of fluoxetine (which treat antidepression only); but it must not be taken in combination with it.


International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 7)
S. M. Aburawi and S. A. Baayo, "Behavior Effect of Fluoxetine in Presence of Selenium Using Albino Mice", International Journal of Pharmacology, Phytochemistry and Ethnomedicine, Vol. 7, pp. 1-8, 2017
Online since:
Jun 2017

[1] T. Vos, Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990-2010. A Systematic Analysis for the Global Burden of Disease Study, Lancet. 380 (2012) 2163-2196.

DOI: 10.3410/f.719894686.793525441

[2] L. Robinson, J. Segal, M. Smith, Depression in men, Helpguide Organization, 2013. Available: http: /www. helpguide. org.

[3] M. Smith, L. Robinson, J. Segal, Depression in older adults and the elderly, 2013. Available: http: /www. helpguide. org.

[4] C. Chilvers et al., Antidepressant drugs and generic counselling for treatment of major depression in primary care: randomised trial with patient preference compares antidepressants and counseling, Brit. Med. J. 322(7289) (2001) 772-775.

DOI: 10.1136/bmj.322.7289.772

[5] R.A. Harrigan, W.J. Brady, ECG abnormalities in tricyclic antidepressant ingestion, The American Journal of Emergency Medicine. 17(4) (1999) 387-393.

DOI: 10.1016/s0735-6757(99)90094-3

[6] S.H. Preskorn et al., Selective serotonin reuptake inhibitors, in: Antidepressants: past, present and future, Verlag Berlin, Heidelberg, 2004, pp.241-262.

DOI: 10.1007/978-3-642-18500-7_9

[7] S.B. Goldhaber, Trace element risk assessment: essentiality vs. toxicity, Regulatory Toxicology and Pharmacology. 38(2) (2003) 232-242.

DOI: 10.1016/s0273-2300(02)00020-x

[8] C.D. Thomson, Assessment of requirements for selenium and adequacy of selenium status: a review. Eur. J. Clin. Nutr. 58(3) (2004) 391-402.

DOI: 10.1038/sj.ejcn.1601800

[9] G.F. Combs, W.P. Gray, Chemo preventive agents: selenium, Pharmacology and Therapeutics. 79(3) (1998) 179-192.

[10] K. Weber, Selenium and depression, 2012. Available: http: /www . boost mood. com.

[11] S. Jayesh, A. Ashish, T. Chandrabhanub, Evaluation of anwtidepressant like activity of curcumin and its combination with fluoxetine and imipramine: An acute and chronic study, Acta. Poloniae Pharmaceutica. 68 (2011) 769-775.

[12] B. Naghibi, F. Rayatnia, Co-administration of sub effective anxiolytic doses of diazepam and hydroxyzine in elevated zero-maze in mice, Psychiatry Investig. 8 (2010) 169-173.

DOI: 10.4306/pi.2011.8.2.169

[13] C.R. Jesse et al., Evidence for the involvement of the serotonergic 5-HT2A/C and 5-HT3 receptors in the antidepressant-like effect caused by oral administration of bis selenide in mice, Progress in Neuro-Psychopharmacology and Biological Psychiatry. 34(2) (2010).

DOI: 10.1016/j.pnpbp.2009.11.023

[14] Z. Rogoz, G. Skuza, B. Legutko, Repeated treatment with mirtazapine induces brain-derived neurotrophic factor gene expression in rats, J. Physiol. Pharmacol. 56 (2005) 661-671.

[15] A. Swayeh et al., Nitroparacetamol exhibits anti-inflammatory and anti-nociceptive activity, Brit. J. Pharmacol. 130 (2000) 1453-1456.

DOI: 10.1038/sj.bjp.0703509

[16] C. Vinader-Caerols et al., Acute effects of maprotiline on learning, anxiety, activity and analgesia in male and female mice, Acta Neurobiologiae Experiment. 66(1) (2006) 23-31.

[17] S. Kumar, A. Sharma, Anti-anxiety activity studies on homoeopathic formulations of Turnera aphrodisiaca ward, Evidence-based Complementary and Alternative Medicine. 2(1) (2005) 117-119.

DOI: 10.1093/ecam/neh069

[18] R.J. Rodgers, Animal models of anxiety: where next? Behav. Pharmacol. 8 (1997) 477-496.

[19] S.M. Aburawi, Study of neuro chemical mechanisms involved in tolerance and physical dependence to traizolam in experimental animals, Ph.D. Thesis, Cairo University, (1999).

[20] S. Ueki, H. Sugano, A quantitative method for continuous recording of spontaneous activity in small animals, Jap. J. Pharmacol. 17 (1967) 82-89.

DOI: 10.1254/jjp.17.82

[21] L.B. Rojeck, Z. Kalodera, I. Samarzija, The antidepressant activity of Hypericum perforatum L. measured by two experimental methods on mice, Acta Pharma. 54 (2004) 157-162.

[22] J.F. Cryan, M.E. Page, I. Luki, Noradrenergic lesions differentially alter the antidepressant like effects of reboxetine in a modified forced swim test, Eur. J. Pharmacol. 436 (2002) 179-205.

DOI: 10.1016/s0014-2999(01)01628-4

[23] W.D. Brandy, Selenium and depression studies, 2013. Available: http: /www. livestrong. com.

[24] A. Holmes et al., Behavioral profile of wild mice in the elevated plus-maze test for anxiety, Physiol. Behav. 71 (2000) 509-516.

DOI: 10.1016/s0031-9384(00)00373-5

[25] J. Podhorna, R.E. Brown, Strain differences in activity and emotionality do not account for differences in learning and memory performance between C57BL/6 and DBA/2 mice, Genes. Brain. Behav. 1 (2002) 96-110.

DOI: 10.1034/j.1601-183x.2002.10205.x

[26] B. Demouliere, F. Chenu, M. Bourin, Forced swimming test in mice: a review of antidepressant activity, Psychopharmacol. 177 (2005) 245-255.

DOI: 10.1007/s00213-004-2048-7

[27] R.D. Porsolt, A. Bertin, M. Jalfre, Behavioral despair in mice: a primary screening test for antidepressants, Arch. Int. Pharmacodyn. Ther. 229(2) (1977) 327-336.

[28] J.P. Reneric, I. Lucki, Antidepressant behavioral effects by dual inhibition of monoamine reuptake in the rat forced swimming test, Psychopharmacol. 136 (1998) 190-197.

DOI: 10.1007/s002130050555

[29] C. Michael et al., Integrated Pharmacology (2nd ed. ), C.V. Mosby, (2002).

[30] A. McIntosh, A. Cohen, N. Turnbull, Clinical guidelines and evidence review for panic disorder and generalized anxiety disorder, National Institute for Clinical Excellence, 2004, pp.165-166.

[31] M.J. Rapoport, K.L. Lanctôt, D.L. Streiner, Benzodiazepine use and driving: a meta-analysis, J. Clin. Psychiatry. 70 (2009) 663-673.

[32] G. Ghisleni, V. Kazlauckas, Diphenyl diselenide exerts anxiolytic-like effect in Wistar rats: Putative roles of GABAA and 5HT receptors, Prog. Neuropsycho Pharmacol. Biol. Psychiatry. 32 (2008) 1508-1509.

DOI: 10.1016/j.pnpbp.2008.05.008

[33] T. Wahls, Multiple Sclerosis, 2008. Available: http: /www. terrywahls. com.

[34] C.E.S. Oliveira et al., The antidepressant-like action of a simple selenium-containing molecule, methyl phenyl selenide, in mice, Eur. J. Pharmacol. 690(1) (2012) 119-123.

[35] T. Posser et al., Antidepressant like effect of organo selenium compound ebselen in mice; Evidence of the involvement of the monoaminergic system, Eur. J. Pharmacol. 602(1) (2009) 1-5.

[36] M. Kurt, A.C. Arik, S. Celik, The effects of sertraline and fluoxetine on anxiety in the elevated plus-maze test in mice, J. Basic. Clin. Physiol. Pharmaco. 11 (2000) 173-180.

DOI: 10.1515/jbcpp.2000.11.2.173

[37] W.K. Kroeze, L.R. Roth, The molecular biology of 5HT receptors: Therapeutic implications for the interface of mood and psychosis, Biol. Psychiatry. 44 (1998) 1128-1142.

DOI: 10.1016/s0006-3223(98)00132-2

[38] G.B. Varty et al., The Gerbil elevated plus-maze I: Behavioral characterization and pharmacological validation, Neuro Psycho Pharmacol. 27 (2002) 357-370.

DOI: 10.1016/s0893-133x(02)00312-3

[39] R.T. Robinson, B.C. Drafts, J.L. Fisher, Fluoxetine increases GABA(A) receptor activity through a novel modulatory site, J. Pharmacol. Exp. Ther. 304(3) (2003) 978-984.

[40] S. Mora et al., Anxiolytic and antidepressant-like effects of the hydroalcoholic extract from Aloysia polystachya in rats, Pharmacol. Bioch. Behav. 82 (2005) 373-378.

DOI: 10.1016/j.pbb.2005.09.007

[41] K. Lobato et al., α-Tocopherol administration produces an antidepressant-like effect in predictive animal models of depression, Behav. Br. Res. 209(2) (2010) 249-259.

[42] N.B. Sandson, S.C. Armstrong, L. Kelly, An overview of psychotropic drug-drug interactions, Psychosomatics. 46 (2005) 464-494.

DOI: 10.1176/appi.psy.46.5.464

[43] B. Nehru, A. Iyer, Effect of selenium on lead-induced neurotoxicity in different brain regions of adult rats, J. Environ. Pathol. Toxicol. Oncol. 13 (1994) 265-268.

[44] L.M. Zanatta et al., In vivo and in vitro effect of imipramine and fluoxetine on Na+, K+-ATPase activity in synaptic plasma membranes from the cerebral cortex of rats, Braz. J. Med. Biolog. Res. 34 (2001) 1265-1269.

DOI: 10.1590/s0100-879x2001001000005
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