Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

IJPPE > Volume 6 > Pre-Exposure to Dennettia tripetala Ethanolic...
< Back to Volume

Pre-Exposure to Dennettia tripetala Ethanolic Fruit Extract Prevents Biochemical Alterations in Rats Subsequently Exposed to a Single Dose of Carbon Tetrachloride

Full Text PDF


Dennettia tripetala (DT) is consumed in West Africa as a spice. It is also used in traditional medicine for treating cough, fever and other ailments. Its fruits have been shown to possess phytochemicals with proven antioxidant capabilities. DT roots elicit in vitro antioxidant capabilities similar to that of ascorbic acid. In this study, we evaluated the potency of the ethanol extract of DT fruits in preventing liver damage induced by a single oral administration of Carbon tetrachloride (CCl4). Thirty female Wistar albino rats were randomized into six groups of five animals each: Group A served as control, Groups B-D were given increasing doses (250, 500 and 1000mg/kg b.w respectively) of DT extract for 14 days by gavage; on day 15, they were given CCl4 (3ml/kg bw) by gavage. Group E was given the highest dose of DT for 14 days without CCl4 while Group F was given only CCl4 on day 15. Administration of CCl4 resulted in liver and kidney injury detected as significant increases in plasma ALT, AST, ALP and GGT activities as well as plasma total protein, urea, creatinine, plasma and liver triglyceride and cholesterol as well as liver and kidney malondialdehyde concentrations. CCl4 also caused a reduction in liver and kidney SOD and catalase activities as well as plasma albumin concentration. Pre-treatment with Dennettia tripetala however, significantly prevented damage to the liver and kidney. Under the conditions of this study, Dennettia tripetala shows potential in preventing liver and kidney damage and has no noticeable side effects.


International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 6)
S. O. Iseghohi et al., "Pre-Exposure to Dennettia tripetala Ethanolic Fruit Extract Prevents Biochemical Alterations in Rats Subsequently Exposed to a Single Dose of Carbon Tetrachloride", International Journal of Pharmacology, Phytochemistry and Ethnomedicine, Vol. 6, pp. 8-16, 2017
Online since:
January 2017

[1] L.W. Weber, M. Boll, A. Stampfl, Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model, Crit Rev Toxicol. 33 (2003) 150–136.

[2] L.M. Berger et al., CCl4–induced toxicity in isolated hepatocytes: The importance of direct solvent injury, Hepatology. 6 (1986) 36–45.


[3] M. Al-Yahya et al., Attenuation of CCl4-induced oxidative stress and hepatonephrotoxicity by Saudi Sidr honey in rats, Evid. based compl. Alt. Med. (2013) 1–10.


[4] P. Abraham, G. Wilfred, S.P. Cathrine, Oxidative damage to the lipids and proteins of the lungs, testes and kidney of rats during carbon tetrachloride intoxication, Clinica. Chimica. Acta. 289 (1999) 177–179.


[5] S.O. Iseghohi, A review of the uses and medicinal properties of Dennettia tripetala (Pepperfruit), Med. Sci. 3 (2015) 104–111.


[6] B.O. Ejechi, D.E. Akpomedaye, Activity of essential oil and phenolic acid extracts of pepperfruit (Dennettia tripetala G. Baker; Anonaceae) against some food-borne microorganisms, Afr. J. Biotech. 4 (2005) 258–261.

[7] D.E. Okwu, F.N.I. Morah, Mineral and nutritive value of Dennettia tripetala fruits, Fruits. 59 (2004) 437–442.


[8] N.P. Okolie, A. Falodun, O. Davids, Evaluation of the antioxidant activity of the root extract of Pepperfruit (Dennettiatripetala) and its potential for the inhibition of lipid peroxidation, Afr. J. Trad. Compl. Alt. Med. 11 (2014) 221–227.


[9] U.E. Odoh, C.O. Ezugwu, J.C. Dike, The phenolic content and antioxidant effect of the methanol extract of Dennettia tripetala G. Baker (Annonaceae), Planta Med. 80(10) (2014).


[10] M.A. Aderogba, E.O. Akinkunmi, W.T. Mabusela, Antioxidant and antimicrobial activities of flavonoid glycosides from Dennettia tripetala G. Baker leaf extract, Nig. J. Nat. Pro. Med. 15 (2011) 49–52.


[11] E. Nwachukwu, J. Osuji, Evaluation of plant extracts for antifungal activity against Sclerotium rolfsii causing cocoyam cormel rot in storage, Res. J. Agr. Bio. 6 (2008) 784–787.

[12] O.O. Anyaele, A.A.S. Amusan, Toxicity of hexanolic extract of Dennettia tripetala (G. Baker) on larvae of Aedes aegypti (l), Afr. J. Biomed. Res. 6 (2003) 49–53.


[13] B. O. Ejechi, O. E. Nwafor, F. J. Okoko, Growth inhibition of Tomato-rot fungi by phenolic acids and essential oil extracts of pepper fruit, Food Res. Int. 32 (1999) 395–399.

[14] I.A. Oyemitan et al., Antinociceptive and anti-inflammatory effects of essential oil of Dennettia tripetala G. Baker (Annonaceae) in rodents, Afr. J. Trad. Compl. Alt. Med. 5 (2008) 355–362.


[15] A.O. Anaga, I.U. Asuzu, Glucose uptake-enhancing activity of the ethyl acetate extract of Dennettia tripetala in 3T3-L1 adipocytes, J. Compl. Integr. Med. (2011).


[16] A.O. Anaga, I.U. Asuzu, Antihyperglycaemic properties of the ethyl acetate extract of Dennettia tripetala in diabetic rats, J. Compl. Int. Med. 7(1) (2010).


[17] H.P. Misra, I. Fridovich, The role of superoxide anion in the autooxidation of epinephrine and simple assay for superoxide dismutase, J. Biol. Chem. 247 (1972) 3170–3175.

[18] L. Goth, A simple method for determination of serum catalase activity and revision of reference range, Clin. Chim. Acta. 196 (1991) 143–151.


[19] J.A. Buege, S.D. Aust, Microsomal lipid peroxidation, Methods enzymol. 52 (1978) 302–310.

[20] A. Singh, T.K. Bhat, O.P. Sharma, Clinical biochemistry of hepatotoxicity, J. Clinic. Toxicol. S4 (2011) 1–19.

[21] A. Ihemeje et al., Biochemical evaluation of Pepperfruit (Dennettia tripetala) and its use as substitute for ginger in zobo drink production, Acad. Res. Int. 4 (2013) 513–521.

[22] M. Boll et al., Mechanism of carbon tetrachloride induced hepatotoxicity. Hepatocellular damage by reactive CCl4 metabolites, Z. Naturforsch. 56 (2001) 649–659.

[23] B. Fromentry, D. Pessayre, Inhibitional mitochondrial β-oxidation as a mechanism of hepatotoxicity, Pharmacol. Ther. 67 (1995) 101–154.

[24] H. Kato, Y. Nakazawa, The effect of CCl4 on the enzymatic hydrolysis of cellular triacylglycerol in adult rat hepatocytes in primary monolayer culture, Bio. Pharm. 36 (1987) 1807–1814.


[25] A.A.A. Khalaf et al., Comparative study on the protective effect of some antioxidants against CCl4 hepatotoxicity in rats, Egypt. J. Nat. Tox. 6 (2009) 59–82.

[26] J.S. Owen, Extra hepatic cell membrane lipid abnormalities and cellular dysfunction in liver disease, Drugs. 40 (1990) 73–83.


[27] C.E. Jahn et al., Lipoprotein abnormalities in primary biliary cirrhosis association with hepatic lipase inhibition as well as altered cholesterol esterification, Gastroenterology. 89 (1985) 1266–1278.

[28] U.Y. Sanzgiri et al., Uptake, distribution, and elimination of carbon tetrachloride in rat tissues following inhalation and ingestion exposures, Tox. Appl. Pharm. 143 (1997) 120–129.


[29] F. Jaramillo-Juarez et al., Acute renal failure induced by carbon tetrachloride in rats with hepatic cirrhosis, Annals of Hepatology. 7 (2008) 331–338.

Show More Hide
Cited By:

[1] S. Iseghohi, N. Orhue, "Aqueous extract of Dennettia tripetala ameliorates liver and kidney damage caused by multiple exposures to carbon tetrachloride", Clinical Phytoscience, Vol. 3, 2017