Feature of Experimental Breast Cancer Induced by Intermammary Administration of N-Methyl-N-Nitrosourea in Wistar Rat

Lykov, Alexander P.; Kabakov, Alexei V.; Kazakov, Oleg V.; Bondarenko, Natalia A.; Poveshchenko, Olga V.; Rayter, Tatyana V.; Poveshchenko, Alexander F.; Strunkin, Dmitri N.; Bogashev, Sergei S.; Pokushalov, Eugenii A.; Konenkov, Vladimir I.

doi:10.18052/www.scipress.com/IJPPE.6.54

IJPPE > Volume 6 > Feature of Experimental Breast Cancer Induced by...

< Back to Volume

Feature of Experimental Breast Cancer Induced by Intermammary Administration of N-Methyl-N-Nitrosourea in Wistar Rat

Full Text PDF

Abstract:

Abstract. Breast cancer is the leading cause of cancer death in women worldwide due to the complicated etiology involving both genetic and environmental factors. BC was induced by N-Methyl-N-Nitrosourea administration intermammary in Wistar female. Studied frequency of development of NMU-induced BC, phenotype of BC cells, phenotype of MSC from mammary gland tissue, quantity and quality of immunocompetent cells, expression of miRNAs, hormone levels and cytokines were done for estimate sensitivity to chemotherapy. We showed that MNU-induced BC developed in 90%, immunohistochemical similar to human luminal A type of BC with higher proliferation activity. We estimated significantly increased count of BC-MSC with CD45+CD90-, and CD45+CD90+, and CD45-CD90+ phenotype in animal with MNU-induced BC. Chemotherapy caused changes in quality and quantity of immunocompetent cells, decrease of prolactin and luteinizing hormone levels, and some changes in expression of pro-oncogenic or tumor-suppressing miRNAs. In MNU-induced BC animal were observed increased serum levels of pro-inflammatory cytokines. In regional lymph nodes found increased count of macrophages and presence of tumor cells. Intermammary administration of NMU possessed to breast cancer development with similarities to human luminal B phenotype. This MNU-induced breast cancer may serve as a model to study efficiency of chemotherapy.

Info:

Periodical:

International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 6)

Pages:

54-71

DOI:

https://doi.org/10.18052/www.scipress.com/IJPPE.6.54

Citation:

A. P. Lykov et al., "Feature of Experimental Breast Cancer Induced by Intermammary Administration of N-Methyl-N-Nitrosourea in Wistar Rat", International Journal of Pharmacology, Phytochemistry and Ethnomedicine, Vol. 6, pp. 54-71, 2017

Online since:

January 2017

Authors:

Alexander P. Lykov, Alexei V. Kabakov, Oleg V. Kazakov, Natalia A. Bondarenko, Olga V. Poveshchenko, Tatyana V. Rayter, Alexander F. Poveshchenko, Dmitri N. Strunkin, Sergei S. Bogashev, Eugenii A. Pokushalov, Vladimir I. Konenkov

Keywords:

Breast Cancer, Breast Cancer Cells Phenotype, Chemotherapy, Cytokines, miRNA, Panagen, Sex Hormones, Tumor-Associated Mesenchymal Cells

Export:

RIS, BibTeX, Text

Distribution:

Open Access

This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

C. Hui et al., MicroRNA-34 and microRNA-21 play roles in the chemopreventive effects of 3, 6-dihydroxyflavone on 1-methyl-1-nitrosourea-induced breast carcinogenesis, Breast Cancer Res. 14 (2012) 80-91.

B. Pula et al., Immunohistochemical Characterization of N-Methyl-N-Nitrosourea-induced Mammary Tumours of Sprague-Dawley Rats, In vivo. 27 (2013) 793-802.

M. P. Thorpe et al., Breast tumors induced by n-methyl-n-nitrosourea are damaging to bone strength, structure, and mineralization in the absence of metastasis in rats, J. Bone and Mineral Res. 26 (2011) 769-776.

A.D. Papaconstantinou et al., Gene expression profiling in the mammary gland of rats treated with 7, 12-dimethylbenz[a]anthracene, Int. J. Cancer. 118 (2006) 17-24.

H. Jaafar, F. Mohmad Irdis, S. N. Mohd Nafi, The association between phenotype and size of breast tumors induced by 1-methyl-1-nitrosourea (MNU) injection in rats, Med. Sci. Monit. 15 (2009) 129-134.

E. El-Abd et al., Histopathological characteristics of breast cancer and evaluation of ER alpha and Her-2neu using immunohistochemical and RT-PCR techniques, Alexandria J. Med. 50 (2014) 275-282.

S. Bernichtein, P. Touraine, V. Goffin, New concepts in prolactin biology, J. Endocrinol. 206 (2010) 1-11.

G. N. Farhat et al., Sex hormone levels and risks of estrogen receptor-negative and estrogen receptor-positive breast cancers, J. Nat. Cancer Inst. 103 (2011) 562-570.

P. Szychta et al., TSH receptor antibodies have predictive value for breast cancer - retrospective analysis, Thyroid Res. 6 (2013) 8-15.

J. Zhou et al., Serum follicle-stimulating hormone level is associated with human epidermal growth factor receptor type 2 and Ki67 expression in post-menopausal females with breast cancer, Oncol Let. 6 (2013) 1128-1132.

P.S. Waters et al., Relationship between circulating and tissue microRNAs in a murine model of breast cancer, PLoS ONE (2012).

M.A. Al-Rakan et al., Breast stromal fibroblasts from histologically normal surgical margins are pro-carcinogenic, J. Pathol. 231 (2013) 457-465.

B.G. Cuiffo et al., MSC-regulated microRNAs converge on the transcription factor FOXP2 and promote breast cancer metastasis, Cell Stem Cell. 15 (2014) 762-774.

C. Zhang et al., Mesenchymal stem cells derived from breast cancer tissue promote the proliferation and migration of the MCF- cell line in vitro, Oncol. Let. 6 (2013) 1577-1582.

A. R. Chochieva, The study of the influence of letrosole on some factors of hormonal and metabolic status under experimental carcinogenesis of the mammary gland, Siberian J. Oncol. 1 (2008) 100-102.

W. Wang et al., Prognostic and predictive value of Ki-67 in triple-negative breast cancer, Information on www. impactjournals. com/oncotarget.

C. Katanov et al., Regulation of the inflammatory profile of stromal cells in human breast cancer: prominent roles for TNF-α and the NF-κB pathway, Stem Cell Res. Therap. 6(1) (2015) 87.

L. Lacerda et al., Mesenchymal stem cells mediate the clinical phenotype of inflammatory breast cancer in a preclinical model, Breast Cancer Res. 17 (2015).

L. Usha et al., Mesenchymal stem cells develop tumor tropism but do not accelerate breast cancer tumorigenesis in a somatic mouse breast cancer model, PLoS ONE. 8 (2013).

M. Zhao et al., Mesenchymal stem cells in mammary adipose tissue stimulate progression of breast cancer resembling the basal-type, Cancer Biol. Therapy. 13 (2012) 782-792.

A.S. Proskurina et al., Results of multicenter double-blind placebocontrolled phase II clinical trial of Panagen preparation to evaluate its leukostimulatory activity and formation of the adaptive immune response in patients with stage II-IV breast cancer, BMC Cancer. 15 (2015).

S. Bernichtein, P. Touraine, V. Goffin, New concepts in prolactin biology, J. Endocrinol. 206 (2010) 1-11.

L.M. Nitze et al., Reevaluation of the proposed autocrine proliferative function of prolactin in breast cancer, Breast Cancer Res. Treat. 142 (2013) 31-44.

P. Szychta et al., TSH receptor antibodies have predictive value for breast cancerretrospective analysis, Thyroid Res. 16 (2013).

K.B. Reddy, MicroRNA (miRNA) in cancer, Cancer Cell Intern. 15 (2015).

S.D. Selcuklu et al., Regulatory interplay between miR-21, JAG1 and 17beta-estradiol (E2) in breast cancer cells, Biochem. Biophys. Res. Comm. 423 (2012) 234-239.

K. Gombos et al., Effect of N-Methyl-N-Nitrosurea on microRNA expression in CBA/CA mice, J. Environ. Occup Sci. 1 (2012) 77-82.

A.P. Lykov et al., The levels of microRNA in the lymph in experimental models of breast cancer, International J. Applied and Fundamental Research. 6 (2015) 445-452.

M.Y. Shah, G.A. Calin, MicroRNAs miR-221 and miR-222: a new level of regulation in aggressive breast cancer, Genome Med. 3(8) (2011) 56.

U. Gezer et al., Abundant circulating microRNAs in breast cancer patients fluctuate considerably during neoadjuvant chemotherapy, Oncol. Let. 8 (2014) 845-848.

Y. Yao et al., 5-FU and ixabepilone modify the microRNA expression profiles in MDA-MB453 triple-negative breast cancer cells, Oncol. Let. 7 (2014) 541-547.

M. Esquivel-Velazquez et al., The role of cytokines in breast cancer development and progression, J. Interferon Cytokine Res. 35 (2015).

A. Nicolini, A. Carpi, G. Rossi, Cytokines in breast cancer, Cytokine Growth Factor Rev. 17 (2006) 325-337.

A. Purohit, S.P. Newman, M.J. Reed, The role of cytokines in regulating estrogen synthesis: implications for the etiology of breast cancer, Breast Cancer Res. 4 (2002) 65-69.

M.E. Dzodzikova et al., Mast cells of the mammary gland and of the regional lymph node in rats with breast cancer induced by N-Methyl-N-Nitrosourea, Morfologiia. 128(4) (2004) 6064.

Show More Hide

Cited By:

This article has no citations.