Subscribe

Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

IJPPE > Volume 5 > Antioxidant Activity of Indian Propolis - An In...
Antioxidant Activity of Indian Propolis - An<i> In Vitro </i>Evaluation
< Back to Volume

Antioxidant Activity of Indian Propolis - An In Vitro Evaluation

Full Text PDF

Abstract:

Antioxidants from the natural products are essential to prevent the progression of free radical mediated diseases. In the present study, ethanol extract of propolis collected from different geographical origin were evaluated for their free radical scavenging potential by employing different in-vitro assays such as DPPH, ABTS, Nitric oxide and Hydrogen peroxide. All the tested samples contained considerable amount of total phenols and vitamin C content. In the entire assay, the percentage of inhibition increased with the increase in concentration. Among the propolis samples collected, the highest activity was found in Tamil Nadu, Kerala, Karnataka and Haryana. The difference in the antioxidant activity level was obtained from the assay may reflect a relative difference in the ability of antioxidant compounds to scavenge different free radicals in the extract. Phenols and vitamin C are the major contributors to antioxidant activity in propolis. The propolis from these locations may be of considerable interest in preventing the ill effects of excessive free radical generation in the human body.

Info:

Periodical:
International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 5)
Pages:
79-85
DOI:
https://doi.org/10.18052/www.scipress.com/IJPPE.5.79
Citation:
S. Ramnath and S. Venkataramegowda, "Antioxidant Activity of Indian Propolis - An In Vitro Evaluation", International Journal of Pharmacology, Phytochemistry and Ethnomedicine, Vol. 5, pp. 79-85, 2016
Online since:
October 2016
Authors:
Shubharani Ramnath, Sivaram Venkataramegowda
Keywords:
ABTS, Antioxidants, Bee-Propolis, DPPH, Hydrogen Peroxide, Nitric Oxide
Export:
RIS, BibTeX, Text
Distribution:
Open Access

Creative Commons License
This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

[1] V. Bankova, S.L. Castro, M.C. Marcucci, Propolis: recent advances in chemistry and plant origin, Apidologie. 31(1) (2000) 3–15.

[2] Y.K. Park, S.M. Alenkar, C.L. Aguiar, Botanical origin and chemical composition of Brazilian propolis, J. Agric. Food. Chem. 50 (9) (2002) 2502–2506.

[3] M. Velikova et al., Antibacterial ent-kaurene from Brazilian propolis of native stingless bees, Fitoterapia. 71 (2000) 693-696.

[4] H. Miyataka et al., Evaluation of propolis. I. Evaluation of Brazilian and Chinese propolis by enzymatic and physico-chemical methods, Biol. Pharm. Bull. 20(5) (1997) 496-501.

[5] D. Naik, H.S. Vaidya, B.C. Behera, Antioxidant Properties of Volatile Oil of Indian Propolis, J. ApiProducts and ApiMedical Science. 1(4) (2009) 110-120.

[6] R.I. Gonzales Corcho et al., Hepatoprotective effects of propolis extract on carbon tetrachloride-induced liver injury in rats, Phytoth. Res. 9 (1995) 114-117.

[7] V. Dimov et al., Immunomodulatory action of propolis. Influence on anti-infectious protection and macrophage function, Apidologie. 22 (1991) 155-162.

[8] A.H. Banskota, Y. Tezuka, S. Kadota, Recent progress in pharmacological research of propolis, Phytoth. Res. 15(7) (2001) 561-571.

[9] R. Shubharani, V. Sivaram, B.R. Kishore, In-vitro cytotoxicity of Indian bee propolis on cancer cell lines, Int. J. Pharma. Bio. Sci. 5(4) (2014) 698 – 706.

[10] G.A. Burdock, Review of the biological properties and toxicity of bee propolis (propolis), Food. Chem. Toxicol. 36 (4) (1998) 347-363.

[11] D. Vijay Wagh, Review Article Propolis: A Wonder Bees Product and Its Pharmacological Potentials, Adv. Pharmacol. Sci. 2013 (2013) Article ID 308249.

[12] C.M. Mihai et al., Antioxidant Capacity of Transylvanian Propolis, Bulletin UASVM Animal Science and Biotechnologies. 67(1-2) (2010) 132-138.

[13] Y.H. Yen, C.H. Shih, C.H. Chang, Effect of adding ascorbic acid and glucose on the antioxidative properties during storage of dried carrot, Food Chem. 107 (2008) 265–272.

[14] C.C. Chang et al., Estimation of total flavonoids content in propolis by two complementary colorimetric methods, J. Food. Drug. Anal. 10(3) (2002) 178-182.

[15] A.K. Goyal, S.K. Middha, A. Sen, Evaluation of the DPPH radical scavenging activity, total phenols and antioxidant activities in Indian wild Bambusa vulgaris Vittata, methanolic leaf extract, J. Nat. Pharm. 1 (2010) 40-45.

[16] R. Re et al., Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radic. Biol. Med. 26(9-10) (1999) 1231-1237.

[17] Y. Sumanont, Y. Murakami, M. Tohda, Evaluation of the Nitric Oxide Radical Scavenging Activity of Manganese Complexes of Curcumin and Its Derivative, Biol. Pharm. Bull. 27(2) (2004) 170-173.

[18] R.J. Ruch, S.J. Cheng, J.E. Klaunig, Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea, Carcinogen. 10(6) (1989) 1003-1008.

DOI: https://doi.org/10.1093/carcin/10.6.1003

[19] S. Padayatty et al., Vitamin C as an antioxidant: Evaluation of its role in disease prevention, J. Am. Coll. Nutr. 22(1) (2003) 18-35.

[20] C. Rice-Evans, Flavonoid antioxidants, Curr. Med. Chem. 8(7) (2001) 797–807.

[21] M.P. Kahkonen et al., Antioxidant activity of plant extracts containing phenolic compounds, J. Agric. Food. Chem. 47(10) (1999) 3954–3962.

[22] T. Hatano et al., Effects of the interaction of tannins with co-existing substances. VI. Effects of tannins and related polyphenols on superoxide anion radical, and on 1, 1-diphenyl-pierylhydrazyl radical, Chem. Pharm. Bull. 37 (1989).

[23] K. Sowndhararajan et al., In vitro antioxidant characteristics of different parts of Melothria maderaspatana (L. ) COGN. Int. J. Pharm. Pharm. Sci. 2(3) (2010) 117-123.

[24] X.C. Li et al., Antioxidant activity and mechanism of protochatechuic acid in-vitro, J. Functional Food in Health and Disease. 1 (2011) 232-244.

[25] M. J. Miller et al., Amelioration of chronic ileitis by nitric oxide synthase inhibition, J. Pharm. Exp. Ther. 264 (1993) 11-16.

Show More Hide