Effects of Oral Administration of D-002 (Beeswax Alcohols) on Histological and Functional Outcomes in a Rat Model of Antigen-Induced Arthritis: Preliminary Study

Noa Puig, Miriam; Mendoza Castaño, Sarahí; Ferreiro, Rosa Mas; Valle Clara, Maikel; Mendoza Hernansez, Nilda

doi:10.18052/www.scipress.com/IJPPE.5.60

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Effects of Oral Administration of D-002 (Beeswax Alcohols) on Histological and Functional Outcomes in a Rat Model of Antigen-Induced Arthritis: Preliminary Study

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Abstract:

D-002, a mixture of higher aliphatic beeswax alcohols, has been shown to display anti-inflammatory effects associated with the dual inhibition of ciclooxygenase and 5-lipoxygenase. Oral D-002 supplementation has been effective in experimental osteoarthritis, ameliorating all features of joint histopathology. Clinical studies have demonstrated that D-002 reduces osteoarthritis symptoms. However, D-002 effects on experimental models of rheumatoid arthritis (RA) have not been evaluated. To investigate whether D-002 improves histopathological and functional outcomes in a rat model of antigen-induced arthritis. First experiment. Rats were randomized into a negative vehicle-control (sham) and four groups injected with complete Freund's adjuvant (CFA): a positive vehicle-control, three treated with D-002 (50, 200 and 400 mg/kg/day) for 21 days. Second experiment. Rats were randomized into a sham and four CFA-injected groups: a positive vehicle-control, two treated with D-002 (25 and 100 mg/kg/day), one with methorexate (MTX) (0.3 mg/kg) for 28 days. Arthritis severity was evaluated by bodyweight loss, decreased exploratory activity and histological changes of tarsal joint and spleen samples in both experiments, except the exploratory activity, assessed only in the first one. CFA injection decreased the bodyweight and the exploratory activity, and induced infiltration of mononuclear cells, pannus formation and vascularity in the tarsal joint of positive control rats. These changes were significantly and markedly ameliorated by D-002 as compared to the positive control. MTX also reversed CFA-induced changes. The reduction of the infiltration of mononuclear cell with D-002 400 mg/kg was greater (80.9%) than with MTX (66.8%), but effects on other variables were similar. No abnormalities in spleen samples of D-002-treated groups were detected. This is the first report demonstrating the efficacy of oral treatment with D-002 in a rat model of antigen-induced arthritis. Results suggest that D-002 could help manage RA, but confirmation of such potential benefit requires extensive further research.

Info:

Periodical:

International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 5)

Pages:

60-68

DOI:

https://doi.org/10.18052/www.scipress.com/IJPPE.5.60

Citation:

M. Noa Puig et al., "Effects of Oral Administration of D-002 (Beeswax Alcohols) on Histological and Functional Outcomes in a Rat Model of Antigen-Induced Arthritis: Preliminary Study", International Journal of Pharmacology, Phytochemistry and Ethnomedicine, Vol. 5, pp. 60-68, 2016

Online since:

October 2016

Authors:

Miriam Noa Puig, Sarahí Mendoza Castaño, Rosa Mas Ferreiro, Maikel Valle Clara, Nilda Mendoza Hernansez

Keywords:

Antigen-Induced Arthritis, Beeswax Alcohol, Histological Changes, Rat

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Open Access

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Creative Commons Attribution 4.0 International License

References:

[1] M. Cutolo, G.D. Kitas, PL. van Riel, Burden of disease in treated rheumatoid arthritis patients: going beyond the joint, Semin. Arthritis Rheum. 43 (2014) 479-488.

[2] E. Salgado, J.R. Maneiro, New therapies for rheumatoid arthritis, Med. Clin. (Barc). 13 (2014) 841-845.

[3] H. Radner, J.S. Smolen, D. Aletaha, Impact of comorbidity on physical function in patients with rheumatoid arthritis, Ann. Rheu. Dis. 69 (2010) 536–541.

[4] E. Lindqvist et al., Ten year outcome in a cohort of patients with early rheumatoid arthritis: health status, disease process, and damage, Ann. Rheu. Dis. 61(2002) 1055–1059.

[5] H. Radner, J.S. Smolen, D. Aletaha, Remission in rheumatoid arthritis: benefit over low disease activity in patient reported outcomes and costs, Arthritis Res. Ther. 16 (2014) R56.

[6] CL. Overman et al., Change of psychological distress and physical disability in patients with rheumatoid arthritis over the last two decades, Arthritis Care Res. (Hoboken). 66(5) (2014) 671-678.

[7] H. To, Rheumatoid arthritis, Nihon Rinsh. 71 (2013) 2141-2145.

[8] D. Palmer, Y. El Mledany, Treat-to-target: a tailored treatment approach to rheumatoid arthritis, Br. J. Nurs. 22 (2013) 312-318.

[9] O. Ethgen et al., What do we know about the safety of corticosteroids in rheumatoid arthritis? Curr. Med. Res. Opin. 29 (2013) 1147-1160.

[10] P. Cipriani et al., Methotrexate in rheumatoid arthritis: optimizing therapy among different formulations. Current and emerging paradigms, Clin. Ther. 36 (2014) 427-435.

DOI: https://doi.org/10.1016/j.clinthera.2014.01.014

[11] LJ. Crofford, Use of NSAIDs in treating patients with arthritis, Arthritis Res. Ther. 15(3) (2013) S2.

[12] EL. Massó González et al., Variability among non-steroidal antiinflammatory drugs in risk of upper gastrointestinal bleeding, Arthritis and Rheumatism. 15 (2010) 1592–1601.

DOI: https://doi.org/10.1002/art.27412

[13] M. Hegen et al., Utility of animal models for identification of potential therapeutics for rheumatoid arthritis, Ann. Rheum. Dis. 67 (2008) 1505-1515.

[14] DL. Asquith et al., Animal models of rheumatoid arthritis, Eur. J. Immunol. 39 (2009) 2040-(2044).

[15] A. Beresniak et al., Interest of modelling in rheumatoid arthritis, Clin. Exp. Rheumatol. 4 Suppl. 73 (2012) S96.

[16] D. Carbajal et al., Anti-inflammatory activity of D-002: an active product isolated from beeswax, Prostaglandins Leukot Essent Fatty Acids. 59 (1998) 235-238.

[17] D. Carbajal et al., Effect of D-002 on models of acute inflammation, IJPSRR 21 (2013) 62-67.

[18] Y. Ravelo et al., Effects of single oral and topical administration of D-002 (beeswax alcohols) on xylene induced ear edema in mice, Latin American Journal of Pharmacy. 29 (2010) 1451-1454.

[19] Y. Ravelo et al., Evaluation of antiinflammatory and antinociceptive effects of D-002 (beeswax alcohols), J. Nat. Med. 65 (2010) 330-335.

[20] Y. Perez et al., Effect of D-002 on 5-lypooxygenase activity in vitro, Rev. Cubana Farm. 46 (2012) 259–266.

[21] Y. Pérez et al., Inhibition of ciclooxygenase and 5-lipooxygenase enzymes by D-002 (beeswax alcohols), Top Current Nutraceutical Res. 12 (2014) 13-18.

[22] S. Mendoza et al., Effects of D-002 on formaldehyde-induced osteoarthritis in rats. IOSR J. Pharm. 3 (2013) 9-12.

[23] S Mendoza et al., Ameliorating effects of D-002, a mixture of beeswax alcohols, on monosodium iodoacetate-induced osteoarthritis in rats, Int. J. Pharm. Sci. Rev. Res. 19 (2013) 10-15.

[24] I. Rodríguez et al., Effects of D-002, a mixture of beeswax alcohols, on osteoarthritis symptoms: a randomized placebo-controlled study, IOSR J. Pharm. 2 (2012) 1–9.

[25] R. Puente et al., Evaluation of the effect of D-002, a mixture of beeswax alcohols, on arthritic symptoms, The Korean journal of internal medicine. 29 (2014) 191–202.

[26] K. Bauerova et al., Utilization of adjuvant arthritis model for evaluation of new approaches in rheumatoid arthritis therapy focused on regulation of immune processes and oxidative stress, Interdisc. Toxicol. 4 (2011) 33–39.

[27] A. Al-Abda et al., Nimesulide improves the disease modifying anti-rheumatic profile of methotrexate in mice with collagen-induced arthritis, European J. Pharmacol. 644 (2010) 245-250.

[28] Y. Haruna et al., Fluvastatin reverses endothelial dysfunction and increased vascular oxidative stress in rat adjuvant-induced arthritis. Arthritis & Rheumatism. 56 (2007) 1827-1835.

[29] U. Heilborn et al., Spontaneous nociceptive behaviour in female mice with Freund's complete adjuvant- and carrageenan-induced monoarthritis, Brain Res. 27 (2007) 143-149.

[30] D. Carbajal et al., Efecto del D-004 sobre patrones conductuales en roedores, Rev. CENIC Ciencias Biológicas. 38 (2007) 12-17.

[31] L. Mosekilde, C. Danielsen, U.B. Knudsen, The effect of aging and ovariectomy on the vertebral bone mass and biomechanical properties of mature rats, Bone. 14 (1993) 1-6.

[32] A. Omoto et al., Copper chelation with tetrathiomolybdate suppresses adjuvant-induced arthritis and inflammation-associated cachexia in rats, Arthritis Res. Ther. 7 (2005) R1174-R1182.

[33] AM. Bendele, Animal models of rheumatoid arthritis, J. Musculoskel Neuron Interact. 1 (2001) 377-385.

[34] R. Jaimik et al., Ameliorative effect of Bambusa arundinacea against adjuvant arthritis-with special reference to bone erosion and tropical splenomegaly, J. of Drug Delivery and Therap. 2 (2012) 141-145.

DOI: https://doi.org/10.22270/jddt.v2i3.150

[35] Y. Ozkan et al., Oxidative status in rheumatoid arthritis, Clin. Rheumatol. 26(1) (2007) 64–68.

[36] A. Nakajima et al., Identification of clinical parameters associated with serum oxidative stress in patients with rheumatoid arthritis, Mod. Rheumatol. 24 (2014) 926–930.

[37] M. Veselinovic et al., Oxidative stress in rheumatoid arthritis patients: relationship to diseases activity, Mol. Cell Biochem. 391 (2014) 225-232.

[38] RM. El-Sayed, YM. Moustafa, MF. El-Azab, Evening primrose oil and celecoxib inhibited pathological angiogenesis, inflammation, and oxidative stress in adjuvant-induced arthritis: novel role of angiopoietin-1, Inflammopharmacology. 5 (2014).

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