Evaluation of In Vitro Antimicrobial and Anti-Inflammatory Potentials of Crude Methanolic Extracts of Excoecaria agallocha (Leaves)

Rahman, Md. Abdur; Hussain, Md. Saddam; Millat, Md. Shalahuddin; Moghal, Md. Mizanur Rahman

doi:10.18052/www.scipress.com/IJPPE.5.25

IJPPE > Volume 5 > Evaluation of In Vitro Antimicrobial and...

< Back to Volume

Evaluation of In Vitro Antimicrobial and Anti-Inflammatory Potentials of Crude Methanolic Extracts of Excoecaria agallocha (Leaves)

Full Text PDF

Abstract:

Excoecaria agallocha plant (Leaves) was analyzed to explore In Vitro antimicrobial and membrane stabilizing activities as a crude methanolic extract. Antimicrobial activity was performed against a wide range of Gram positive(+Ve) and Gram negative(-Ve) bacteria by using disc diffusion method and various methanolic extracts of leaves of E.agallocha was tested for determining membrane stabilizing activity at hypotonic solution and heat induce condition and standard acetyl salicylic acid (0.10 mg/mL) was employed as standard. the zones of inhibition created was found to be 1.3 cm at a concentration of 100 µL/ disc in case of Gram negative(-Ve) bacteria Salmonella typhi. The results obtained were compared with that of a standard Ampicillin (10 µL) and imipenem (10µL), penicillin (10µl), cefoxitine(30µl). Crude methanolic extract of 10mg/ml concentration showed maximum value of 17.67±0.0102 and 18.92±0.086% respectively under hypotonic solution and heat induced condition. Thus the result suggest that, crude methanolic extracts of E.agallocha possessed slight to moderate antimicrobial and membrane stabilizing properties.

Info:

Periodical:

International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 5)

Pages:

25-33

DOI:

https://doi.org/10.18052/www.scipress.com/IJPPE.5.25

Citation:

M. A. Rahman et al., "Evaluation of In Vitro Antimicrobial and Anti-Inflammatory Potentials of Crude Methanolic Extracts of Excoecaria agallocha (Leaves)", International Journal of Pharmacology, Phytochemistry and Ethnomedicine, Vol. 5, pp. 25-33, 2016

Online since:

October 2016

Authors:

Md. Abdur Rahman, Md. Saddam Hussain, Md. Shalahuddin Millat, Md. Mizanur Rahman Moghal

Keywords:

Antimicrobial, Euphorbiaceae, Excoecaria agallocha, Leaves, Membrane Stabilization

Export:

RIS, BibTeX, Text

Distribution:

Open Access

This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

[1] M.A.A. Al-Bari, M.A. Sayeed, M.S. Rahman, Characterization and antimicrobial activities of a phenolic acid derivative produced by Streptomyces bangladeshiensis, a novel species collected in Bangladesh, Res. J. Medicine and Med. Sci. 1 (2006).

[2] J. Bauer, R. Rojas, B. Bustamante, Antimicrobial activity of selected Peruvian medicinal plants, J. Ethnopharmacol. 88 (2003) 199- 204.

[3] B.H. Shahidi, Evaluation of antimicrobial properties of Iranian medicinal plants against Micrococcus luteus, Serratiamarcescens, Klebsiella pneumonia and Bordetella bronchoseptica, Asian J. Plant Sci. 3 (2004) 82-86.

DOI: https://doi.org/10.3923/ajps.2004.82.86

[4] D. Runyoro et al., Screening of Tanzanian medicinal plants for anti-Candida activity, BMC Complement Altern. Med. 6 (2006) 11.

DOI: https://doi.org/10.1186/1472-6882-6-11

[5] C.A.O. Anosike, Obidoa, L.U. Ezeanyika, Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum), DARU J Pharm Sci. 20 (2012) 76.

DOI: https://doi.org/10.1186/2008-2231-20-76

[6] J.B. Perianayagam, S.K. Sharma, S.K. Pillai, Anti-inflammatory activity of Trichodesmaindicum root extract in experimental animals, J. Ethnopharmacol. 104 (2006) 410-414.

DOI: https://doi.org/10.1016/j.jep.2005.08.077

[7] J.R. Vane, R.M. Botting, New insight into the mode of action of anti-inflammatory drugs, Inflamm. Res. 44 (1995) 1-10.

[8] J.M. Munro, R.S. Cotran, The pathogenesis of atherosclerosis: atherogenesis and inflammation, Lab Invest. 58 (1988) 249-261.

[9] R.W. Alexander, Inflammation and coronary artery disease, N Engl J Med. 331 (1994) 468-469.

[10] R. Ross, The pathogenesis of atherosclerosis: a perspective for the 1990s, Nature. 362 (1993) 801-809.

[11] H. Hossain et al., Anti-inflammatory activity, total flavonoids and tannins content from the ethanolic extract of Spilanthes paniculata leaf growing in Bangladesh, Int. J. Pharm. 2 (2012) 271–277.

[12] K.D. Tripathi, Essentials of medical pharmacology, JP Medical Ltd., New Delhi, (2013).

[13] P.N. Bennett, M.J. Brown, Clinical pharmacology, Churchill Livingstone, New Delhi, (2005).

[14] B. Halliwell, J.M.C. Gutteridge, C.E. Cross, Free radicals, antioxidants, and human disease: where are we now? J Lab Clin Med. 119 (1992) 598–620.

[15] A. Ghani, Medicinal plants of Bangladesh, Asiatic society of Bangladesh, Dhaka. 37 (1998) 54-56.

[16] A. Goldstein. Principles of drug action-the basis of pharmacology, 2 (1974) 736-755.

[17] R.A. Gossline, The status of natural products in the American Pharmaceutical Market, Lloydia. 25(4) (1962) 241.

[18] S. Myers, hytochemical methods (a guide to modern techniques to plant analysis), Champan and Hall, USA, Edition. 3 (1982) 335-337.

[19] D.J. Newman, G.M. Cragg, K.M. Snader, Natural products as sources of new drugs over the period 1981-2002, J. Nat. Prod. 66 (2003) 1022-1037.

DOI: https://doi.org/10.1021/np030096l

[20] A.W. Bauer et al., Antibiotic susceptibility testing by standardized single method, Amer. J. Clin. Path. 45(4) (1966) 493-496.

[21] J. Ahmed et al., Determination of chemical groups and investigation of anthelmintic, cytotoxic, and antibacterial activities of leaves of Cinnamomum Tamala (Family: Lauraceae), Int. J. Pharmamedix India. 1 (2013) 222– 232.

[22] M.A. Sikder et al., In vitro antioxidant, reducing power, free radical scavenging and membrane stabilizing activities of Spilanthes calva, Bangladesh Pharm. J. 13 (2010) 63–67.

[23] FAO, Trade in Medicinal Plants, Economic and Social Department, Food and Agriculture Organization of the United Nations, Rome, Italy, (2004).

[24] M. Deepa, C.K. Padmaja, Preliminary phytochemical analysis and thin layer chromatography of the extracts of Excoecaria agallocha L., International Journal of Pharmaceutical Sciences and Research. 5(10) (2014) 4535-4542.

[25] M. Ferrali et al., Iron release and membrane damage in erythrocytes exposed to oxidizing agents, phenylhydrazine, divicine and isouramil, Biochemical Journal. 285 (1992) 295-301.

DOI: https://doi.org/10.1042/bj2850295

[26] O. Augusto, K.L. Kunze, P.R. Montellano, Nphenylprotoporphyr information in the haemogolobinphenylhydrazine reaction, The Journal of Biological Chemistry. 257 (1982) 6231-6241.

[27] B. Halliwell, J.R.S. Hoult, D.R. Bllake, Oxidants, inflammation and anti-inflammatory drugs, The FASEB Journal. 2 (1988) 501-508.

[28] S.R.J. Maxwell, Prospects for the use of anti-oxidant therapies, Drugs. 49 (1995) 345-361.

[29] R.M. Perenz et al., Anti-inflammatory activity of the bark of Hippocratea excels, Journal of Ethnopharmacology. 47 (1995) 85-90.

[30] U.A. Shinde et al., Membrane stabilizingactivity–a possible mechanism of action for the antiinflammatoryactivity of Cedrus deodara wood oil, Fitoterapia. 70 (1999) 251-257.

DOI: https://doi.org/10.1016/s0367-326x(99)00030-1

Show More Hide

Cited By:

This article has no citations.