A Study on the Therapeutic Ability of Gut Microbiota upon V. parahaemolyticus Infected Farm Prawn

Reangasamy, Nandakumar; Muthaiyan, Rajikkannu; Paramasivam, Raghu; Arumugam, Deva; Radha, Baburajan; Krishnan, Prabhakaran; Viveganandan, Masilamni

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A Study on the Therapeutic Ability of Gut Microbiota upon V. parahaemolyticus Infected Farm Prawn

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Abstract:

Glycogen content of the prawn muscles from the three groups showed increasing trend after 30 days of feed supplementation. In control animals recorded maximum of 2.2 mg/g, and in B. coagulans supplemented animals it was 4.1 mg/g and 3.6 mg/g in B. firmus supplemented animals increase in glycogen content in relation with feed supplementation with probiotic bacteria was most prominent in prawns. The increase in alpha amylases content may be attributed to the influence of the probiotic strain which possess exoenzyme activity. Maximum colonies were found in B. firmus supplemented animals gut, 962 cfu after 30 days of feed supplementation. Similar trend wasobserved in intestinal microflora of all three groups after infection in the feed supplemented animals. The one way analysis of variance showed significant variation among feed supplementation and treatment. The one way analysis revealed that B. coagulans supplemented group recorded maximum glycogen followed by B. firmus and control groups. Maximum THB count was observed in B. firmus supplemented animals after infecting the animals with V.parahaemolyticus. Alpha amylase activity was maximum in B. firmus supplemented animals was recorded. Significant results could be observed in one way analysis of variance.

Info:

Periodical:

International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 2)

Pages:

20-29

DOI:

https://doi.org/10.18052/www.scipress.com/IJPPE.2.20

Citation:

N. Reangasamy et al., "A Study on the Therapeutic Ability of Gut Microbiota upon V. parahaemolyticus Infected Farm Prawn", International Journal of Pharmacology, Phytochemistry and Ethnomedicine, Vol. 2, pp. 20-29, 2016

Online since:

May 2016

Authors:

Nandakumar Reangasamy, Rajikkannu Muthaiyan, Raghu Paramasivam, Deva Arumugam, Baburajan Radha, Prabhakaran Krishnan, Masilamni Viveganandan

Keywords:

Amylase Activity, B. coagulans, B. firmus, Gut Analysis

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Open Access

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Creative Commons Attribution 4.0 International License

References:

[1] C.R. Lavilla-Pitogo, C.L. Baticados, E.R. Cruz-Lacierda, L. de la Pena, Occurrence of luminous bacteria disease of Penaeus monodon larvae in the Philippines, Aquaculture, 91 (1990) 1-13.

DOI: https://doi.org/10.1016/0044-8486(90)90173-k

[2] P. Jirvanichpaisal, T. Miyazaki, Histopathology, Biochemistry and pathogenicity of Vibrio harveyi infecting black tiger shrimp Penaeus monodon, J. Aquat. An. Health. 6 (1994) 27-35.

[3] J. F Guillot, Probiotic feed additives, J. Vet. Pharmacol. Ther. 26 (2003) 52-55.

[4] D.R.W. Griffith, Microbiology and the role of probiotics in Ecuadorian shrimp hatcheries. In: P. Lavens, E. Jaspers, I. Roelants, (Eds. ), Larvi' 95-Fish and Shellfish Larviculture Symposium. Special Publications, European Aquaculture Society, Gent, Belgium, 24 (1995).

[5] D. Garriques, G. Arevalo, An evaluation of the production and use of a live bacterial isolate to manipulate the microbial flora in the commercial production of Penaeus vannamei postlarvae in Ecuador. In: Browdy, C.L., Hopkins, J.S. (Ed. ), Swimming Through Troubled Water. Proceedings of the Special Session on Shrimp Farming, Aquaculture'95. World Aquaculture Society, Baton Rouge, Louisiana, USA, (1995).

[6] D.J.W. Moriarty, Control of luminous Vibrio species in penaeid aquaculture ponds, Aquaculture, 164 (1998) 351-358.

DOI: https://doi.org/10.1016/s0044-8486(98)00199-9

[7] S.W. Rengpipat, S. Phianphak, Piyatiratitivorakul, P. Menasveta, Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth, Aquaculture, 167 (1998) 301-313.

DOI: https://doi.org/10.1016/s0044-8486(98)00305-6

[8] M. Maeda, I.C. Liao, Effect of bacterial population on the growth of a prawn larva, Penaeus monodon, Bull. Natl. Res. Inst. Aquac. 21 (1992) 25-29.

[9] K. Nogami, M. Maeda, Bacteria as biocontrol agents for rearing larvae of the Crab Portunustrituber culatus, Can. J. Fish. Aquat. Sci. 49 (1992) 2373-2376.

DOI: https://doi.org/10.1139/f92-261

[10] V. Tanasomwang, T. Nakai, Y. Nishimura, K. Muroga, Vibrio-inhibiting marine bacteria isolated from black tiger shrimp hatchery, Fish. Pathol. 33(5) (1998) 459-466.

DOI: https://doi.org/10.3147/jsfp.33.459

[11] S. Rengpipat, S. Rukpratanporn, S. Piyatiratitivorakul, P. Menasaveta, Immunity enhancement in black tiger shrimp (Penaeus monodon) by a probiont bacterium (Bacillus S11), Aquaculture, 191 (2000) 271-288.

DOI: https://doi.org/10.1016/s0044-8486(00)00440-3

[12] B. Gomez-Gil, L. Tron-mayen, A. Roque, J.F. Turnbull, V. Inglis, A.L. Guerra-Flores, Species of Vibrio isolated from hepatopancreas, haemolymph and digestive tract of a population of healthy juvenile Penaeus vannamei, Aquaculture, 163 (1998) 1-9.

DOI: https://doi.org/10.1016/s0044-8486(98)00162-8

[13] L.G. Verschuere, P. Rombaut, W. Sorgeloos, Verstraete, Probiotic bacteria as biological control agents in aquaculture, Microbiol. Mol. Biol. Rev. 64 (4) (2000) 655-671.

DOI: https://doi.org/10.1128/mmbr.64.4.655-671.2000

[14] S.N. Mohanty, S.K. Swain, S.D. Tripathi, Growth and survival of rohu spawn fed on liver based diet, J. Inland. Fish. Soc. India. 25(2) (1993) 41-45.

[15] P. K Mohanty, S.K. Dash, P.K. Mishra, A.S. Murty, Heat and momentum fluxes over Chilika: a tropical lagoon, Ind. J. Marine Sci. 25 (1996) 184-188.

[16] O.P. Sharma, S.K.S. Bhukhar, Effect of Aquazyn-TM-1000, a probiotic on the water quality and growth of Cyprinus carpio var. communis (L. ), Ind. J. Fish. 47(3) (2000) 209-213.

[17] Y.J.H. Wang, R.L. Cheng, Y.Q. Edwards, K.G. He, Z.S. Kong, J.Y. An, M.J. Wu, X.D. Kelly, C.A.D. ykoski, Li, The Holocene Asian Monsoon: Links to solar changes and North Atlantic climate, Science, 308 (2005) 854-857.

DOI: https://doi.org/10.1126/science.1106296

[18] Y.B. Wang, Z.R. Xu, Effect of probiotics for common carp (Cyprinus carpio) based on growth performance and digestive enzyme activities. Anim. Feed. Sci. Technol. 127 (2005) 283-292.

DOI: https://doi.org/10.1016/j.anifeedsci.2005.09.003

[19] A.G.J. Tacon, Standard methods for the nutrition and feeding of farmed fish and shrimp, Washington DC, Argent Laboratories Press, 1990, p.454.

[20] P. A West, R.R. Colwell, Identification and classification overview. In R. R. Colwell (ed. ), Vibrios in the environment. John Wiley & Sons, Inc., New York, 1984, pp.285-363.

[21] J.H. Roe, The determination of sugar blood in blood and spinal fluid with anthrone reagent, J. Bio. Chem. 242 (1955) 424-428.

[22] F.J. Gatesoupe, The use of probiotics in aquaculture, Aquaculture, 180 (1999) 147-165.

DOI: https://doi.org/10.1016/s0044-8486(99)00187-8

[23] A.C. Campbell, J.A. Buswell, The intestinal microflora of farmed Dover sole (Solea solea) at different stages of fish development, J. Appl. Bacteriol. 55 (1983) 215-223.

DOI: https://doi.org/10.1111/j.1365-2672.1983.tb01318.x

[24] G.H. Hansen, E. Strom, J.A. Olafsen, Effects of deferent holding regimens on the intestinal microflora of herring (Clupeahar engus) larvae, Appl. Environ. Microbiol. 58 (1992) 461-470.

[25] P.D. Munro, A. Barbour, T.H. Birkbeck, Comparison of the gut bacterial flora of start-feeding larval turbot reared under different conditions, J. Appl. Bacteriol. 77 (1994) 560-566.

DOI: https://doi.org/10.1111/j.1365-2672.1994.tb04402.x

[26] E. Ringo, T.H. Birkbeck, P.D. Munro, O. Vadstein, K. Hjelmeland, The effect of early exposure to Vibrio pelagius on theaerobic bacterial flora of turbot, Scophthalmus maximus (L. ) larvae. J. Applied. Bacteriol. 81 (1996) 207-211.

DOI: https://doi.org/10.1111/j.1365-2672.1996.tb04502.x

[27] J.L. Johnson, C.F. Phelps, C.S. Cummins, J. London, F. Gasser, Taxonomy of the Lactobacillus acidophilus group. Int. J. System. Bacteriol. 30 (1980) 53-68.

DOI: https://doi.org/10.1099/00207713-30-1-53

[28] H.H. Sung, T.Y. Hong, The gram negative bacterial flora in hepato pancreas of giant freshwater prawn (Macrobrachium rosenbergii): antibiotics sensitivities and production of extracellular products. J. Fish. Soc. Taiwan. 24 (1997) 211-223.

[29] D.J.W. Moriarty, Interactions of microorganisms and aquatic animals, particularly the nutritional role of the gut flora. In: (Le´sel R. Ed. ), Microbiology in Poecilotherms. Elsevier, Amsterdam, (1990) 217-222.

[30] D.J.W. Moriarty, The role of microorganisms in aquaculture ponds. Aquaculture. 151 (1997) 333-349.

DOI: https://doi.org/10.1016/s0044-8486(96)01487-1

[31] N.J.P. Gournier-Chateau, I. Larpent, J.L. Castellanos, Larpent, Les Probiotics and Alimentation Animal and Human. Technique Documentation Lavoisier, Paris, (1994) 192.

[32] J.D. Clements, Detection of spontaneous synaptic events with an optimally scaled template. Biophys. J. 73(1) (1997) 220-229.

DOI: https://doi.org/10.1016/s0006-3495(97)78062-7

[33] D.J.W. Moriarty, Disease Control in shrimp aquaculture with probiotic bacteria. Micrbial. Inter. Aquaculture. (1996) 1-7.

[34] I.E. Saeed, K. Sopian, Z. ZainolAbidin, Drying kinetics of Roselle (Hibiscus sabdariffa L. ): dried in constant temperature and humidity chamber. Proc. SPS 2006. Edited by Muchtar. 29th-30th August. Permata, Bangi, S.D.E., Malaysia: (2006).

[35] X.F. Wang, A.S. Auler, R.L. Edwards, H. Cheng, Ito, M. Solheid, Interhemispheric anti-phasing of rainfall during the last glacial period, Quat. Sci. Rev. 25 (2006) 3391-3403.

DOI: https://doi.org/10.1016/j.quascirev.2006.02.009

[36] D. Lovett, D. Felder, Ontogenic change in digestive enzyme activity of larval and postlarval white shrimp Penaeus setiferus (Crustacea, Decapoda, Penaeidae). Biol. Bull. 178 (1990) 144-159.

DOI: https://doi.org/10.2307/1541973

[37] M.S. Kamarudin, D.A. Jones, L. Vay, A.Z. Abidin, Ontogenetic change in digestive enzyme activity during larval development of Macrobrachium rosenbergii. Aquaculture, 123 (1994) 323-333.

DOI: https://doi.org/10.1016/0044-8486(94)90068-x

[38] X. Ding, Z.J. Li, Y.Q. Chen, H.Z. Lin, Y.Y. Yang, K. Yang, Effects of probiotics on growth and activities of digestive enzymes of Penaeus vannamei. J. Fish. Sci. China. 11 (2004) 580-584.

[39] S. Ziaei-Nejad, M.H. Rezaei, G.A. Takami, D.L. Lovett, A. Mirvaghefi, M. Shakouri, The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus. Aquaculture, 252 (2006).

DOI: https://doi.org/10.1016/j.aquaculture.2005.07.021

[40] L. Fang, B. Lee, Ontogenic changes in digestive enzymes in Penaeus monodon. Comp. Biochem. Physiol. 103(B) (1992) 1033–1037.

[41] J. Gamboa-Delgado, C. Molina-Poveda, C. Cahu, Digestive enzyme activity and food ingestion in juvenile shrimp Litopenaeus vannamei (Boone, 1931) as a function of body weight, Aquac Res. 34 (2003) 1403-1411.

DOI: https://doi.org/10.1111/j.1365-2109.2003.00959.x

[42] Rosamma Philip, P. Lakshmanaperumalsamy, Studies on bacterial protease: Time course of growth and protease production at different culture conditions. Fish. Tech. 32(1) (1995) 45-49.

[43] I. Karunasagar, I. Karunasagar, Harvest and post harvest Microbiology of fishes. Ind. J. Microbiol. 31 (1991) 211-229.

[44] G. Reid, The growth potential for dairy probiotics. Inter. Dairy J. 49 (2008) 16-22.

[45] S. Rautava, M. Kalliomaki, M. Isolauri, Probiotics during pregnancy and breastfeeding might confer immunomodulatory protection against atopic disease in the infant. J. Allergy. Clin. Immunol. 109 (2002) 119-121.

DOI: https://doi.org/10.1067/mai.2002.120273

[46] M. Viljanen, E. Savilahti, T. Haahtela, K. Juntunen-Backman, R. Korpela, T. Poussa, T. Tuure, M. Kuitunen, Probiotics in the treatment of atopic eczema/dermatitis syndrome in infants: a double-blind placebo-controlled trial. Allergy. 60(4) (2005).

DOI: https://doi.org/10.1111/j.1398-9995.2004.00514.x

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