Subscribe

Subscribe to our Newsletter and get informed about new publication regulary and special discounts for subscribers!

IJPPE > IJPPE Volume 16 > Antimicrobial and Cytotoxic Properties of Extracts...
< Back to Volume

Antimicrobial and Cytotoxic Properties of Extracts from Plants Traditionally Used in North-East Brazil

Full Text PDF

Abstract:

The north-eastern region of Brazil is home to communities of diverse ethnic origins that still depend significantly on folk medicine, with emphasis on plant extracts. The present work aims at investigating medicinal plants used in the traditional medicine of north-eastern Brazil with an assessment of the antimicrobial profile of their extracts. The antimicrobial activity of 12 extracts from 11 plant species from eight higher plant families traditionally used was evaluated against three human pathogens (Staphylococcus aureus, Escherichia coli, Streptococcus pneumoniae) using the Agar Disk Diffusion and Broth Microdilution assays. The cytotoxic effects were evaluated on human keratinocytes. A hydroalcoholic extract from Anadenanthera colubrina presented the most promising in vitro antimicrobial activity against S. aureus associated with low cytotoxicity towards human keratinocytes. The information gathered in the present study represents a starting point for further research aiming at providing scientific evidence to the empirical usage of medicinal plants in traditional practices.

Info:

Periodical:
International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 16)
Pages:
21-32
Citation:
A. Aschrafi et al., "Antimicrobial and Cytotoxic Properties of Extracts from Plants Traditionally Used in North-East Brazil", International Journal of Pharmacology, Phytochemistry and Ethnomedicine, Vol. 16, pp. 21-32, 2021
Online since:
February 2021
Export:
Distribution:
References:

[1] M. de F. Agra, P.F. de Freitas, J.M. Barbosa-Filho, Synopsis of the plants known as medicinal and poisonous in Northeast of Brazil, Revista Brasileira de Farmacognosia. 17 (2007) 114–140.

DOI: https://doi.org/10.1590/s0102-695x2007000100021

[2] J.S.C. de Araújo et al., Antibacterial activity against cariogenic bacteria and cytotoxic and genotoxic potential of Anacardium occidentale L. and Anadenanthera macrocarpa (Benth.) Brenan extracts, Arch. Oral Biol. 85 (2018) 113–119.

DOI: https://doi.org/10.1016/j.archoralbio.2017.10.008

[3] M. Balouiri, M. Sadiki, S.K. Ibnsouda, Methods for in vitro evaluating antimicrobial activity: A review, Journal of Pharmaceutical Analysis. 6 (2016) 71–79.

DOI: https://doi.org/10.1016/j.jpha.2015.11.005

[4] A.M. Benko-Iseppon et al., Ethnobotanical Uses of the Native Flora from Brazilian North-Eastern Region, in: Medicinal Plants: Biodiversity & Drugs, Rai, M.; Rastrelli, L.; Marinoff, M.; Martinez, J.L.; Cordell, G., New Hampshire, (2012).

DOI: https://doi.org/10.1201/b12527-5

[5] A.M. Benko-Iseppon, S. Crovella, Ethnobotanical bioprospection of candidates for potential antimicrobial drugs from Brazilian plants: state of art and perspectives, Curr. Protein Pept. Sci. 11 (2010) 189–194.

DOI: https://doi.org/10.2174/138920310791112129

[6] L.K. Caesar, N.B. Cech, Synergy and antagonism in natural product extracts: when 1 + 1 does not equal 2, Nat Prod Rep. 36 (2019) 869–888.

DOI: https://doi.org/10.1039/c9np00011a

[7] C.L. Delgobo et al., Gum heteropolysaccharide and free reducing mono- and oligosaccharides of Anadenanthera colubrina, Phytochemistry. 47 (1998) 1207–1214.

DOI: https://doi.org/10.1016/s0031-9422(97)00776-0

[8] P. Díaz Dellavalle et al., Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp, Chilean Journal of Agricultural Research. 71 (2011) 231–239.

DOI: https://doi.org/10.4067/s0718-58392011000200008

[9] M.-H. Doosti, K. Ahmadi, M. Fasihi-Ramandi, The effect of ethanolic extract of Thymus kotschyanus on cancer cell growth in vitro and depression-like behavior in the mouse, J Tradit Complement Med. 8 (2018) 89–94.

DOI: https://doi.org/10.1016/j.jtcme.2017.03.003

[10] R.C. Dutra et al., Medicinal plants in Brazil: Pharmacological studies, drug discovery, challenges and perspectives, Pharmacol. Res. 112 (2016) 4–29.

[11] T.E.C. on A.S.T. EUCAST, European Society of Clinical Microbiology and Infectious Disease, Antimicrobial susceptibility testing EUCAST disk diffusion method. Version 7.0, (2019).

[12] O.C. Gómez, J.H.H. Luiz, Endophytic fungi isolated from medicinal plants: future prospects of bioactive natural products from Tabebuia/Handroanthus endophytes, Applied Microbiology and Biotechnology. 102 (2018) 9105–9119.

DOI: https://doi.org/10.1007/s00253-018-9344-3

[13] I.M. van der Heijden et al., Comparison of disc diffusion, Etest and broth microdilution for testing susceptibility of carbapenem-resistant P. aeruginosa to polymyxins, Annals of Clinical Microbiology and Antimicrobials. 6 (2007) 8.

DOI: https://doi.org/10.1186/1476-0711-6-8

[14] W.P. Jones, A.D. Kinghorn, Extraction of Plant Secondary Metabolites, in: S.D. Sarker, L. Nahar (Eds.), Natural Products Isolation, Humana Press, Totowa, NJ, 2012: p.341–366.

DOI: https://doi.org/10.1007/978-1-61779-624-1_13

[15] T. Juwita, I. Melyani Puspitasari, J. Levita, Torch Ginger (Etlingera elatior): A Review on its Botanical Aspects, Phytoconstituents and Pharmacological Activities, Pak. J. Biol. Sci. 21 (2018) 151–165.

DOI: https://doi.org/10.3923/pjbs.2018.151.165

[16] S. Kim, R. Kubec, R.A. Musah, Antibacterial and antifungal activity of sulfur-containing compounds from Petiveria alliacea L, J Ethnopharmacol. 104 (2006) 188–192.

DOI: https://doi.org/10.1016/j.jep.2005.08.072

[17] R. de F. Lima et al., Antimicrobial and Antiproliferative Potential of Anadenanthera colubrina (Vell.) Brenan, Evidence-Based Complementary and Alternative Medicine. 2014 (2014) 1–7.

[18] Ministério da Saúde, Política nacional de plantas medicinais e fitoterápicos, 1a. ed, Ministério da Saúde, Secretaria de Ciência, Tecnologia e Insumos Estratégicos, Departamento de Assistência Farmacêutica, Brasília, DF, Brazil, (2006).

DOI: https://doi.org/10.1590/0103-1104201711217

[19] Ministério da Saúde, MS elabora Relação de Plantas Medicinais de Interesse ao SUS, Agencia da Saude, Brazil, (2009).

[20] Ministério da Saúde, Programa nacional de plantas medicinais e fitoterápicos., Ministério da Saúde, Secretaria de Ciência, Tecnologia e Insumos Estratégicos, Departamento de Assistência Farmacêutica e Insumos Estratégicos, Brasília, DF, Brazil, (2009).

DOI: https://doi.org/10.1590/0103-1104201711217

[21] M.P. Moretão et al., Induction of secretory and tumoricidal activities in peritoneal macrophages activated by an acidic heteropolysaccharide (ARAGAL) from the gum of Anadenanthera colubrina (Angico branco), Immunology Letters. 93 (2004) 189–197.

DOI: https://doi.org/10.1016/j.imlet.2004.03.021

[22] A. Ochoa Pacheco et al., In vitro antimicrobial activity of total extracts of the leaves of Petiveria alliacea L. (Anamu), Brazilian Journal of Pharmaceutical Sciences. 49 (2013) 241–250.

DOI: https://doi.org/10.1590/s1984-82502013000200006

[23] B.-S. Park et al., Antibacterial activity of Tabebuia impetiginosa Martius ex DC (Taheebo) against Helicobacter pylori, J Ethnopharmacol. 105 (2006) 255–262.

DOI: https://doi.org/10.1016/j.jep.2005.11.005

[24] J.G. Park et al., TabetriTM (Tabebuia avellanedae Ethanol Extract) Ameliorates Osteoarthritis Symptoms Induced by Monoiodoacetate through Its Anti-Inflammatory and Chondroprotective Activities, Mediators of Inflammation. 2017 (2017) 1–14.

DOI: https://doi.org/10.1155/2017/3619879

[25] G.M. Rishton, Natural Products as a Robust Source of New Drugs and Drug Leads: Past Successes and Present Day Issues, The American Journal of Cardiology. 101 (2008) S43–S49.

DOI: https://doi.org/10.1016/j.amjcard.2008.02.007

[26] L.F.R. Sampaio, ed., Política nacional de práticas integrativas e complementares no SUS: atitude de ampliação de acesso, 1a. ed, Ministério da Saúde, Secretaria de Atenção à Saúde, Departamento de Atenção Básica, Brasília, DF, Brazil, (2006).

DOI: https://doi.org/10.11606/t.7.2015.tde-13052015-103633

[27] V.S. dos Santos et al., Metabolomics as a tool for understanding the evolution of Tabebuia sensu lato, Metabolomics. 13 (2017).

[28] L.C.N. da Silva et al., Anti-Staphylococcus aureus action of three Caatinga fruits evaluated by electron microscopy, Natural Product Research. 27 (2013) 1492–1496.

DOI: https://doi.org/10.1080/14786419.2012.722090

[29] A. Sofowora, E. Ogunbodede, A. Onayade, The role and place of medicinal plants in the strategies for disease prevention, Afr J Tradit Complement Altern Med. 10 (2013) 210–229.

DOI: https://doi.org/10.4314/ajtcam.v10i5.2

[30] J.O. Suberu et al., Anti-Plasmodial Polyvalent Interactions in Artemisia annua L. Aqueous Extract – Possible Synergistic and Resistance Mechanisms, PLoS ONE. 8 (2013) e80790.

DOI: https://doi.org/10.1371/journal.pone.0080790

[31] D. da S. Trentin et al., Potential of medicinal plants from the Brazilian semi-arid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestyles, Journal of Ethnopharmacology. 137 (2011) 327–335.

DOI: https://doi.org/10.1016/j.jep.2011.05.030

[32] D.S. Trentin et al., Tannins Possessing Bacteriostatic Effect Impair Pseudomonas aeruginosa Adhesion and Biofilm Formation, PLoS ONE. 8 (2013) e66257.

DOI: https://doi.org/10.1371/journal.pone.0066257

[33] C. Urueña et al., Petiveria alliacea extracts uses multiple mechanisms to inhibit growth of human and mouse tumoral cells, BMC Complementary and Alternative Medicine. 8 (2008).

DOI: https://doi.org/10.1186/1472-6882-8-60

[34] WHO, WHO traditional medicine strategy: 2014-2023, (2013).

[35] Q.-W. Zhang, L.-G. Lin, W.-C. Ye, Techniques for extraction and isolation of natural products: a comprehensive review, Chinese Medicine. 13 (2018).

Show More Hide
Cited By:
This article has no citations.