Development and Validation of HPTLC Method for Quantification of Solanesol in Various Parts of Nicotiana tabacum Collected from Different Geographical Regions of India

Kaushik, Sunil; Asif, Mohammad

doi:10.18052/www.scipress.com/IJPPE.10.29

IJPPE > IJPPE Volume 10 > Development and Validation of HPTLC Method for...

< Back to Volume

Development and Validation of HPTLC Method for Quantification of Solanesol in Various Parts of Nicotiana tabacum Collected from Different Geographical Regions of India

Full Text PDF

Abstract:

Solanesol is the starting material for many high value biochemicals, including Co-enzyme Q10 and vitamin-K analogues. The aim of the current study was to develop and validate a reliable and fast analytical procedure for the determination of solanesol in Nicotiana tabacum using high-performance thin layer chromatography (HPTLC) method. The method was developed on TLC aluminium plates precoated with silica gel 60F-254 using solvent system hexane: ethyl acetate (5:1, v/v), which gives compact spot of solanesol (Rf value 0.41 ± 0.02). Densitometric analysis of solanesol was carried out in the absorbance mode at 210 nm. The linear regression analysis data for the calibration plot showed good linear relationship with r = 0.9978 with respect to peak area, in the concentration rang 100-5000 ng per spot of solanesol. The limit of detection and quantification were 13 and 30 ng per spot, respectively. The proposed method was applied for quantitative estimation of solanesol in different parts of Nicotiana tabacum from different geographical regions in India, which showed that maximum amount of solanesol was found to be present in leaf sample collected from Karnataka i.e. 3.52 mg/g. Statistical analysis proved that the method is repeatable, selective and accurate for the estimation of solanesol in Nicotiana tabacum.

Info:

Periodical:

International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 10)

Pages:

29-35

DOI:

https://doi.org/10.18052/www.scipress.com/IJPPE.10.29

Citation:

S. Kaushik and M. Asif, "Development and Validation of HPTLC Method for Quantification of Solanesol in Various Parts of Nicotiana tabacum Collected from Different Geographical Regions of India", International Journal of Pharmacology, Phytochemistry and Ethnomedicine, Vol. 10, pp. 29-35, 2018

Online since:

July 2018

Authors:

Sunil Kaushik, Mohammad Asif

Keywords:

High-Performance Thin Layer Chromatography, Nicotiana tabacum, Solanesol, Validation

Export:

RIS, BibTeX, Text

Distribution:

Open Access

This work is licensed under a
Creative Commons Attribution 4.0 International License

References:

[1] R.L. Rowland, P.H. Latimer, J.A. Giles, Flue cured tobacco. Isolation of solanesol an unsaturated alcohol, Journal of the American Chemical Society. 78(18) (1956) 4680-4683.

[2] C. Zhao, Y. Zu, C. Li, Extraction of solanesol from tobacco leaves by bubble column, Chemical Engineering and Processing. 48 (2009) 203-208.

[3] H.Y. Zhou, C.Z. Liu, Microwave-assisted extraction of solanesol from tobacco leaves, J. Chromatogr. A. 1129 (2006) 135-139.

[4] Y.G. Zu et al., A rapid and sensitive LC-MS/MS method for determination of coenzyme Q10 in tobacco leaves, J. Sep. Sci. 29 (2006) 1607-1612.

[5] J.H. Wang et al., Design, synthesis and synergistic effects of novel derivatives of solanesol, Chemical Research in Chinese Universities. 23 (2007) 417-420.

[6] N. Schechter, T. Nishino, H. Rudney, The synthesis of 3-nonaprenyl-4-hydroxybenzoate in rat liver mitochondria: The effect of Ca2+, Mg2+, chelators, aghubinand bacitracin on the activity of p-hydroxybenzoate-polyprenyl transferase, Archives of Biochemistry and Biophysics. 158(1) (1973).

[7] S. Srivastava et al., Novel hybrid natural products derived from solanesol as wound agent, Indian Journal of Chemistry. 48B (2009) 237-247.

[8] H. Suzuki, A. Tomida, T. Nishimura, Cytocidal activity of a synergistic isoprenoid, N-solanesyl -N, N'bis (3,4 -dimethoxybenzyl) ethylenediamine, and its potentiation of anti tumor drugs against multidrug resistant and sensitive cells in vitro, Jpn. J. Cancer Res. 81 (1990).

[9] S. Nyiredy, G.A. Gross, O. Sticher, Minor alkaloids from Nicotiana Tabacum, J. Nat. Prod. 49(6) (1986) 1156-1157.

[10] M. Bentinger et al., Polyisoprenoid epoxides stimulate the biosynthesis of coenzyme Q and inhibit cholesterol synthesis, J. Biol. Chem. 283 (2008) 14645-14653.

[11] C.J. Zhao, Y.G. Zu, C.Y. Li, Distribution of solanesol in Nicotiana tabacum, Journal of Forestry Research. 18 (2007) 69-72.

[12] M.S. Zhang, J.X. Huang, Determination of solanesol in the extracts of tobacco leaves by high performance liquid chromatography, Se Pu = Chinese journal of chromatography. 19(5) (2001) 470-471.

[13] J. Zhao, C. Wang, X. Sun, Determination of solanesol in the extract of tabacco leaves by HPLC, Se Pu = Chinese journal of chromatography. 15(6) (1997) 544-545.

[14] R. F. Severson et al., Gas chromatographic method for the determination of free and total solanesol in tabacco, J. Chromatography. 139 (1978) 269-282.

[15] J. Chen et al., Optimization of HPLC-APCI-MS conditions for the qualitative and quantitative determination of total solanesol in tobacco leaves, J. Sep. Sci. 31 (2008) 137-42.

[16] C. Zhao, C. Li, Y. Zu, Rapid and quantitative determination of solanesol in Nicotiana tabacum by LC-TMS, J. Pharm. Biomed. Anal. 44(1) (2007) 35-40.

[17] ICH. Text on Validation of Analytical Procedures, Harmonized Tripartite Guideline prepared within the International Conference on Harmonization of Technical Requirements for the Registration of Pharmaceuticals for Human Use. ICH-Q2A, Geneva, (1994).

[18] ICH. Text on Validation of Analytical Procedures: Methodology, Harmonized Tripartite Guideline prepared within the International Conference on Harmonization of Technical Requirements for the Registration of Pharmaceuticals for Human Use. ICH-Q2B, Geneva, (1996).

[19] ICH. Draft guidelines on validation of analytical procedures: definition and technology, federal register, Vol 60. IFPMA, Switzerland, (1995).

Show More Hide

Cited By:

This article has no citations.