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Free Radical Scavenging Activity and Antioxidants of Hydrocotyle vulgaris L. (Pennywort): Baseline Study in Developing Biocosmetic-Antidote for Pathological Aging

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Pathological aging due to harmful free radicals and oxidative stress has been a serious threat to human health (Kensella & He, 2008). Thus, the study aimed to evaluate the free radical scavenging activity, antioxidants, essential vitamins, and physicochemical properties of Hydrocotyle vulgaris L. (Pennywort) which could serve as basis in developing a biocosmetic antidote for pathological aging. H.vulgaris L. plant was harvested, air dried and then extracted via soxhlet. UV-vis spectroscopy was used for scavenging activity and antioxidants of the plant while High Performance Liquid Chromatography (HPLC) was applied for the identification of essential vitamins. Phytochemicals, pH and spreadability values of the plant were also tested. Results revealed that H.vulgaris L. has an abundant presence of flavonoid and alkaloid while traced presence of tannin. Good trend for scavenging activity was also observed exhibiting low absorbance of possible free radicals with IC50 of 29.75 and antioxidant activity of 158.13 (Total Phenolics as gallic acid in %w/w). Meanwhile, β-carotene (10.4 mg/kg), Riboflavin (4.08mg/kg), and Vitamins C (70.2mg/kg) and E (26.9mg/kg) were also found in the plant. Non-irritating pH levels of 5.7 and 7.14 for 50% and 75% concentrations of the extracts with good spreadability value of 17.51 g∙cm/sec (for 75% concentration) were also obtained. Based on the findings of the study, Pennywort has a very promising therapeutic characteristics with its good scavenging activity and antioxidants. Thus, the development of a biocosmetic product that could serve as an antidote for pathological aging from Pennywort appeared to be very apparent.


International Journal of Pharmacology, Phytochemistry and Ethnomedicine (Volume 10)
R. M. Ureta et al., "Free Radical Scavenging Activity and Antioxidants of Hydrocotyle vulgaris L. (Pennywort): Baseline Study in Developing Biocosmetic-Antidote for Pathological Aging", International Journal of Pharmacology, Phytochemistry and Ethnomedicine, Vol. 10, pp. 1-12, 2018
Online since:
July 2018

[1] R. Amarowicz et al., Free-radical scavenging capacity and antioxidant activity of selected plant species from Canadian prairies, Food Chem. 84 (2003) 551-5562.

[2] K.H. Cheeseman, T.F. Slater, An introduction to free radical Biochemistry, British Medical Bulletin. 49(3) (1993) 481–493.

[3] N.P. Das, T.A. Pereira, Effects of flavanoids on thermal autooxidation of palm oil: structure-activity relationship, J. American Oil Chemist Society. 67 (1990) 255-258.

[4] C. DeHaven et al., DNA photoprotection conveyed by sunscreen, Journal of Cosmetic Dermatology. 13(2) (2014) 99-102.

[5] M.A. Ebrahimzadeh et al., Correlation between sun protection factor and antioxidant activity, phenol and flavonoid contents of some medicinal plants, Iranian Journal of Pharmaceutical Research. 13(3) (2014) 1041-1047.

[6] A.E. Hagerman, Tannins as antioxidants, Journal of Agricultural and Food Chemistry. 49 (2002) 4917-4923.

[7] B. Halliwell, J. Gutteridge, Free radicals in biology and medicine, 3rd edition, Clarendon Press, Oxford, UK, (1999).

[8] S. Imam, I. Azhar, Z.A. Mahmood, In-Vitro Evaluation of Sun Protection Factor of Cream Formulation Prepared from Extracts of Musa accuminata L., Psidium gujava, and Pyrus communis L., Asian Journal of Pharmaceutical and Clinical Research. 8(3) (2015).

[9] P. Maisuthissakul, M. Suttajit, R. Pongsawatmanit, Assessment of phenolic content and free radica scavenging capacity of some Thai indigenous plants, Food Chem. 100 (2007) 1409-1418.

[10] F. Maiza-Benabdesselam et al., Antioxidant activities of alkaloid extracts of two Algerian species of fumaria: Fumaria capreolata and Fumaria bastardii, Academy of Chemistry of Globe Publications. 1(2-3) (2007) 28-35.

[11] P.G. Pietta, Flavanoids as antioxidants, Journal of Natural Products. 63(7) (2000) 1035-1042.

[12] B. Poljšak, R. Dahmane, Free radicals and extrinsic skin aging, Dermatology Research and Practice. 2012 (2012) Article ID: 135206.

[13] R.K. Purushotham et al., Preparation and evaluation o/w cream for skin psoriasis, Int. J. Pharm. Bio Sci. 1(3) (2010) 1-11.

[14] S. Saraf, S. Sahu, C.D. Kaur, Comparative measurement of hydration effects of herbal moisturizers, Pharmacognosy Research. 2(3) (2010) 146-51.

[15] S. Schagen et al., Discovering the link between nutrition and skin aging, Dermato Endocrinology. 4(3) (2012) 298-307.

[16] S. Smaoui et al., Cosmetic emulsion from virgin olive oil: Formulation and bio-physical evaluation, Afr. J. Biotech. 11(40) (2012) 9664-967.

[17] M. Younes, Inhibitory action of some flavonoids on enhanced spontaneous lipid peroxidation following glutathione depletion, Planta Medica. 43 (1981) 240-245.

[18] K. Zhou, L. Yu, Effects of extraction solvent on wheat bran antioxidant activity estimation, LWT. 37 (2004) 717-721.

[19] E. Corradini et al., Flavonoids: chemical properties and analytical methodologies ofidentification and quantitation in foods and plants, Natural Product Research. 25(5) (2011) 469-495.

[20] M. Anil, P. Suresh, Determination of free radical scavenging activity in herbal supplement: Chyawanprash, International Journal of Drug Development and Research. 3(1) (2011).

[21] M. Asadujjaman, A. Hossain, U. Kumar-Karmakar, Assessment of DPPH free radical scavenging activity of some medicinal plants, PharmacologyOnLine. 1 (2013) 161-165.

[22] P. Maisuthisakul, M. Suttajit, R. Pongsawatmanit, Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants, Food chemistry. 100(4) (2007) 1409-1418.

[23] F. Pourmorad, S.J. Hosseinimehr, N. Shahabimajd, Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants, African Journal of Biotechnology. 5(11) (2006).

[24] M.S. Stankovic, Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts, Kragujevac J. Sci. 33 (2011) 63-72.

[25] S.M. Mohsen, A.S.M. Ammar, Total phenolic contents and antioxidant activity of corn tassel extracts, Food Chem. 112(3) (2009) 595-598.

[26] A. Weil, Vitamin C Benefits, 2016. Available:

[27] M. Ashoori, A. Saedisomeolia, Riboflavin (vitamin B 2) and oxidative stress: a review, British Journal of Nutrition, 111(11) (2014) 1985-(1991).

[28] C. Nordqvist, All you need to know about beta carotene, 2016. Available:

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